Planta Med 2004; 70(4): 305-309
DOI: 10.1055/s-2004-818940
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Resveratrol Inhibits the Release of Mediators from Bone Marrow-Derived Mouse Mast cells in vitro

Liu Baolin1 , Yoshhiro Inami2 , Hiroyuki Tanaka2 , Naoki Inagaki2 , Munekazu Iinuma2 , Hiroichi Nagai2
  • 1Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing, P. R. China
  • 2Department of Pharmacology of Gifu Pharmaceutical University, Gifu, Japan
Further Information

Publication History

Received: July 30, 2003

Accepted: January 25, 2004

Publication Date:
19 April 2004 (online)

Abstract

Resveratrol is a natural phytoalexin occurring in grapes, vines and peanuts and possesses both antitumor and antioxidation capabilities. Its chemoprotective actions are attributed partially to its anti-inflammation effect. The present study is aimed at checking the inhibitory actions of resveratrol on the release of mediators from bone marrow-derived mouse mast cells (BMMC) in vitro. Mast cells were prepared by isolating bone marrow cells from intact mice femora and culturing them for 4 weeks in the presence of IL-3 and FCS. The release reaction was triggered by IgE or calcium ionophore (A23187). Mediated by IgE, the release of histamine and tumor necrosis factor-α was significantly inhibited by resveratrol at a concentration of 100 μM; IgE-mediated release of leukotrienes and prostaglandin D2 was also strongly suppressed at concentrations of 100 and 10 μM. Also, A23187-mediated release of histamine and leukotrienes release was strongly reduced by resveratrol at concentrations of 100 and 10 μM, respectively. Resveratrol exhibited its behavior without a significant cytotoxic activity against mast cells. In conclusion, resveratrol is a potent non-selective inhibitor of mediator release from activated mast cells and deserves further investigation of its biological modulations.

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Liu Baolin

Department of Pharmacology of Chinese Materia Medica

China Pharmaceutical University

Nanjing 210038

P. R. China

Fax: +86-025-2883499

Email: NJLBL55@SOHU.com