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DOI: 10.1055/s-2004-818942
© Georg Thieme Verlag Stuttgart · New York
Intestinal Anti-Inflammatory Activity of Paepalantine, an Isocoumarin Isolated from the Capitula of Paepalanthus bromelioides, in the Trinitrobenzenesulphonic Acid Model of Rat Colitis
This study has been supported by grants from Instituto de Salud “Carlos III” (PI021732) and from Spanish Ministry of Science and Technology (SAF20002-02592). Research has been performed under the auspices of CYTED (Subprogram X, Project X.10 PIGASTRIN). Luiz C. Di Stasi was a fellowship of CNPq (PDE-200757/01-6)-BrazilPublikationsverlauf
Received: July 23, 2003
Accepted: January 25, 2004
Publikationsdatum:
19. April 2004 (online)
Abstract
The preventative intestinal anti-inflammatory activity of paepalantine, an isocoumarin isolated from the capitula of Paepalanthus bromelioides, was tested in the trinitrobenzenesulphonic acid (TNBS) model of rat colitis. This was performed in two different experimental settings, i. e. when the colonic mucosa is intact or when the mucosa is in process of recovery after an initial insult. The results obtained revealed that the paepalantine pretreatment, at doses of 5 and 10 mg/kg, significantly attenuated the colonic damage induced by TNBS in both situations, as it was evidenced both histologically and biochemically. This beneficial effect was associated with an improvement in the colonic oxidative status, since paepalantine prevented the glutathione depletion that occurred as a consequence of the colonic inflammation. In addition, the intestinal anti-inflammatory effect exerted by this isocoumarin was associated with an inhibition of colonic nitric oxide activity, which is upregulated as a consequence of the inflammatory process. In conclusion, the preventative effect exerted by paepalantine in the TNBS model of rat colitis is probably related with its antioxidant properties.
Abbreviations
AP:Alkaline phosphatase
IBD:Inflammatory bowel disease
GSH:Glutathione
MPO:Myeloperoxidase
NOS:Nitric oxide synthase
TNBS:Trinitrobenzenesulphonic acid
Key words
paepalantine - isocoumarin - capitula of Paepalanthus bromelioides - Eriocaulaceae - antioxidant activity - rat colitis - TNBS
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Julio Galvez, Ph. D.
Department of Pharmacology
School of Pharmacy
University of Granada
18071 Granada
Spain
Telefon: +34-958243889
Fax: +34-958248964
eMail: jgalvez@ugr.es