Ciliary Neurotrophic Factor for Acceleration of Peripheral Nerve Regeneration: An Experimental Study

Jian Zhang; William C. Lineaweaver; Tanya Oswald; Zhengrong Chen; Zhongwei Chen; Feng Zhang

doi:10.1055/s-2004-824891

Journal of Reconstructive Microsurgery, Table of Contents

J Reconstr Microsurg 2004; 20(4): 323-327
DOI: 10.1055/s-2004-824891

Ciliary Neurotrophic Factor for Acceleration of Peripheral Nerve Regeneration: An Experimental Study

Jian Zhang¹ , William C. Lineaweaver¹ , Tanya Oswald¹ , Zhengrong Chen² , Zhongwei Chen² , Feng Zhang¹

¹Division of Plastic Surgery, University of Mississippi, Jackson, MS
²Department of Orthopedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China

Abstract

The objective of this study was to investigate the effect of topically administrated ciliary neurotrophic factor (CNTF) on peripheral nerve regeneration. Sixty-eight Sprague Dawley rats underwent a unilateral sciatic nerve transection and silicon tubulization, with a 10-mm gap between the proximal and distal nerve stumps. Recombinant human CNTF (1 mg/kg) was injected into the rats of the experimental group, while normal saline was injected into the control group animals. Electrophysiologic and histologic studies, including nerve morphometry and electron microscopic observation, were performed at 1, 3, and 4 months postoperatively. HRP tracing was carried out at 3 months postoperatively to label spinal-cord, ventral-horn, and dorsal-root ganglia. The results revealed that CNTF-treated animals showed a higher motor nerve conduction velocity of the sciatic nerve and a higher muscle action potential amplitude of the anterior tibial muscle, compared to the controls (p < 0.01). Nerves repaired with CNTF had larger axon diameter, greater number of axons, and more advanced myelination (p < 0.05). More HRP-labeled motor neurons were also found in the ventral horns of CNTF-treated animals. These results indicate that topical application of CNTF to the injury site potentiates motor nerve axonal regrowth and axon maturation during peripheral nerve regeneration.

KEYWORDS

Ciliary neurotrophic factor (CNTF) - peripheral nerve regeneration

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References

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Feng ZhangM.D. Ph.D.

Division of Plastic Surgery, University of Mississippi Medical Center

2500 North State Street

Jackson, MS 39216