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Horm Metab Res 2004; 36(07): 492-500
DOI: 10.1055/s-2004-825732
Original Clinical

HLA-DRB1*04 and HLA-DQB1*03 Association with the Atrophic but not with the Goitrous Form of Chronic Autoimmune Thyroiditis in a Brazilian Population

D. Engelbrecht Zantut-Wittmann
1   Division of Endocrinology, Department of Internal Medicine, State University of Campinas, Campinas, São Paulo, Brazil
,
L. Persoli
2   Histocompatibility Laboratory-Hemocentro School of Medical Sciences, State University of Campinas, Campinas, São Paulo, Brazil
,
M. A. Tambascia
1   Division of Endocrinology, Department of Internal Medicine, State University of Campinas, Campinas, São Paulo, Brazil
,
E. Fischer
1   Division of Endocrinology, Department of Internal Medicine, State University of Campinas, Campinas, São Paulo, Brazil
,
D. Franco Maldonado
1   Division of Endocrinology, Department of Internal Medicine, State University of Campinas, Campinas, São Paulo, Brazil
,
A. M. Costa
1   Division of Endocrinology, Department of Internal Medicine, State University of Campinas, Campinas, São Paulo, Brazil
,
E. J. Pavin
1   Division of Endocrinology, Department of Internal Medicine, State University of Campinas, Campinas, São Paulo, Brazil
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Erratum zu diesem Artikel:

Correction: HLA-DRB1*04 and HLA-DQB1*03 Association with the Atrophic but not with the Goitrous Form of Chronic Autoimmune Thyroiditis in a Brazilian PopulationHorm Metab Res 2004; 36(07): e1-e1
DOI: 10.1055/a-2083-4743

Abstract

Autoimmune chronic lymphocytic thyroiditis appears in two forms, goitrous and atrophic. The evidence available is not enough to prove that the goitrous precedes the atrophic form, but immunogenetic analysis suggests that they may be distinct entities. The distribution of HLA class II alleles DRB1* and DQB1* was verified in patients from the region of Campinas, São Paulo, Brazil with both forms of thyroiditis. Ninety-one patients with primary hypothyroidism through autoimmune thyroiditis were classified as goitrous - 54 patients, 42.27 ± 11.72 years old, having had hypothyroidism for 8.57 ± 6.63 years - or atrophic - 37 patients, 42.72 ± 12.01 years old, hypothyroidism for 6.73 ± 4.07 years. The distribution of class II alleles was determined, DRB1* and DQB1* were genotyped after purifying DNA blood samples using the DNAzol technique, and the low-resolution PCR-SSP system was utilized for determination of generic alleles. Chi-square and Fisher’s exact test were utilized to compare the distribution frequency of HLA alleles and the significant p-values were subjected to Bonferroni correction. We have demonstrated that the DRB1*04 allele is associated with autoimmune thyroiditis, and that there are genotypic differences regarding the presentation forms with a strong association between DRB1*04 and DQB1*03 and the atrophic form only.

Key words

Autoimmunity - Thyroiditis - HLA typing - Goitrous autoimmune thyroiditis - Atrophic autoimmune thyroiditis


Publikationsverlauf

Eingereicht: 08. September 2003

Angenommen nach Revision: 03. Februar 2004

Publikationsdatum:
11. August 2004 (online)

Georg Thieme Verlag Stuttgart · New York

 

  • References

  • 1 Hayashi Y, Tamai H, Fukata S. A long-term clinical, immunological, and histological follow-up study of patients with goitrous chronic lymphocytic thyroiditis.. J Clin Endocrinol Metab 1985; 61: 1172-1180
    CrossrefPubMedGoogle Scholar
  • 2 Moens H, Farid NR. Hashimoto’s thyroiditis is associated with HLA-Drw3.. N Engl J Med 1978; 299: 133-134
    CrossrefPubMedGoogle Scholar
  • 3 Weissel M, Hofer M, Zasmeta RH, Mayr WR. HLA-DR and Hashimoto’s thyroiditis.. Tis Antig 1980; 16: 256-257
    CrossrefPubMedGoogle Scholar
  • 4 Thomsen M, Ryder LP, Bech K, Bliddal H, Feldt-Rasmussen U, Molholm J, Neilsen H, Svejgaard A. HLA-D in Hashimoto’s thyroiditis.. Tis Antig 1983; 21: 173-175
    CrossrefPubMedGoogle Scholar
  • 5 Wick G, Brezinschek HP, Hala K. Immunoendocrine communication via the hypothalamo-pituitary-adrenal axis in autoimmune diseases.. Endocr Rev 1993; 14: 539-558
    PubMedGoogle Scholar
  • 6 Tomer Y, Davies TF. Infection, thyroid disease, and autoimmunity.. Endocr Rev 1993; 14: 107-118
    PubMedGoogle Scholar
  • 7 Ebner SA, Lueprasitsakul W, Alex S. Iodine content of rat thyroglobulin affects its antigenicity in inducing lymphocytic thyroiditis in the BB/Wor rat.. Autoimmunity 1992; 13: 209-212
    CrossrefPubMedGoogle Scholar
  • 8 Mizukami Y, Michigishi T, Nonomura A. Iodine-induced hypothyroidism: a clinical and histological study of 28 patients.. J Clin Endocrinol Metab 1993; 76: 466-471
    PubMedGoogle Scholar
  • 9 Irvine WJ. The immunology and genetics of autoimmune endocrine disease.. Rose NR, Bigazzi PE, Warner NL. Genetic Control of Autoimmune Disease.. Elsevier; North Holland, New York: 1978: 77-97
    Google Scholar
  • 10 Vargas MT, Briones-Urbina R, Gladman D, Papsin FR, Walfish PG. Antithyroid microsomal autoantibodies and HLA-DR5 are associated with postpartum thyroid dysfunction: evidence supporting an autoimmune pathogenesis.. J Clin Endocrinol Metab 1988; 67: 327-333
    CrossrefPubMedGoogle Scholar
  • 11 Badenhoop K, Schwarz G, Walfish PG, Drummond V, Usadel KH, Bottazzo GF. Susceptibility to thyroid autoimmune disease: molecular analysis of HLA-D identifies new markers for goitrous Hashimoto’s thyroiditis.. J Clin Endocrinol Metab 1990; 71: 1131-1137
    CrossrefPubMedGoogle Scholar
  • 12 Stenszky V, Balazs C, Draszits E, Juhasz F, Kozma L, Balazs G, Farid NR. Association of goitrous autoimmune thyroiditis with HLA-DR3 in eastern Hungary.. J Immunogen 1987; 14: 143-148
    CrossrefPubMedGoogle Scholar
  • 13 Tandon N, Ahang L, Weetman AP. HLA associations with Hashimoto’s thyroiditis.. Clin Endocrinol 1991; 34: 383-386
    CrossrefPubMedGoogle Scholar
  • 14 Thompson C, Farid NR. Postpartum thyroiditis and goitrous (Hashimoto’s) thyroiditis are associated with HLA-DR4.. Immunol Let 1985; 11: 301-303
    CrossrefPubMedGoogle Scholar
  • 15 Shi Y, Zou M, Robb D, Farid NR. Typing for major histocompatibility complex class II antigens in thyroid tissue blocks: association of Hashimoto’s thyroiditis with HLA-DQA 0301 and DQB 0201 alleles.. J Clin Endocrinol Metab 1992; 75: 943-946
    PubMedGoogle Scholar
  • 16 Jenkins D, Mijovic C, Fletcher J, Jacobs KH, Bradwell AR, Barnett AH. Identification of susceptibility for type 1 (insulin-dependent) diabetes by trans-racial gene mapping.. Diabetologia 1992; 33: 387-395
    CrossrefPubMedGoogle Scholar
  • 17 Tamai H, Kimura A, Dong RP, Matsubayashi S, Kuma K, Nagataki S, Sasazuki T. Resistance to autoimmune thyroid disease is associated with HLA-DQ*.. J Clin Endocrinol Metab 1994; 78: 94-97
    PubMedGoogle Scholar
  • 18 Cho BY, Chung JH, Shong YK, Chang YB, Han H, Lee J-B, Lee HK, Koh C-S. A strong association between thyrotropin receptor-blocking antibody-positive atrophic autoimmune thyroiditis and HLA-DR8 and HLA-DQB1*0302 in Koreans.. J Clin Endocrinol Metab 1993; 77: 611-615
    PubMedGoogle Scholar
  • 19 Petrone A, Giorgi G, Mesturino CA, Capizzi M, Cascino I, Nistico L, Osborn J, DiMario U, Buzzetti R. Association of DRB1*04-DQB1*0301 haplotype and lack of association of two polymorphic sites at CTLA-4 gene with Hashimoto’s thyroiditis in an Italian population.. Thyroid 2001; 11: 171-175
    CrossrefPubMedGoogle Scholar
  • 20 Hunt PJ, Marshall SE, Weetman AP, Bunce M, Bells JI, Wass JAH, Welsh KI. Histocompatibility leukocyte antigens and closely linked immunomodulatory genes in autoimmune thyroid disease.. Clin Endocrinol 2001; 55: 491-499
    CrossrefPubMedGoogle Scholar
  • 21 Wallaschofski H, Meyer A, Tuschy U, Lohmann T. HLA-DQA1*0301-associated susceptibility for autoimmune polyglandular syndrome type II and III.. Horm Metab Res 2003; 35: 120-124
    Artikel in Thieme ConnectPubMedGoogle Scholar
  • 22 Bland JM, Altman DG. Multiple significance tests: the Bonferroni method.. Brit Med J 1995; 310: 170-170
    CrossrefPubMedGoogle Scholar
  • 23 Dorak MT. Statistical analysis in HLA and disease association studies.. 2002 http://home.att.net/˜dorak/hla/stat.html
    PubMedGoogle Scholar
  • 24 Fletcher R, Fletcher S, Wagner EH. In: Epidemiologia Clínica. 2a ed., Ed.. Artes Médicas; Porto Alegre-RS, Brasil: 1991
    Google Scholar
  • 25 Vaidya B, Kendall-Taylor P, Pearce SH. The genetics of autoimmune thyroid disease.. J Clin Endocrinol Metab 2002; 87: 5385-5397
    CrossrefPubMedGoogle Scholar
  • 26 Moraes ME, Fernandez-Viña M, Salatiel I, Tsai S, Moraes JR, Stastniy P. HLA class II DNA typing in two Brazilian populations.. Tis Antig 1993; 41: 238-242
    CrossrefPubMedGoogle Scholar
  • 27 Lavard L, Madsen HO, Perrild H, Jacobsen BB, Svejgaard A. HLA class II associations in juvenile Graves’ disease: indication of a strong protective role of the DRB1*0701, DQA1*0201 haplotype.. Tis Antig 1997; 50: 639-641
    CrossrefPubMedGoogle Scholar
  • 28 Chen QY, Huang W, She JX, Baxter F, Volpé R, Maclaren NK. HLA-DRB1*08, DRB1*03/DRB3*0101 and DRB3*0202 are susceptibility genes for Graves’ disease in North American Caucasians, whereas DRB1*07 is protective.. J Clin Endocrinol Metab 1999; 84: 3182-3186
    PubMedGoogle Scholar
  • 29 Buchanan WW, Harden RM. Primary hypothyroidism and Hashimoto’s thyroiditis.. Arch Intern Med 1965; 115: 411
    CrossrefPubMedGoogle Scholar
  • 30 Tunbridge WMG, Evered DC, Hall R, Appleton D, Brewis M, Clark F, Evans JG, Young E, Bird T, Smith PA. The spectrum of thyroid diseases in a community. The Whickham Survey.. Clin Endocrinol 1977; 7: 481
    CrossrefPubMedGoogle Scholar
  • 31 Weetman AP, McGregor AM. Autoimmune thyroid disease: further developments in our understanding.. Endocr Rev 1994; 15: 788-830
    PubMedGoogle Scholar