Zusammenfassung
Kutane T-Zell-Lymphome (CTCL) stellen eine Gruppe lymphoproliferativer Hauterkrankungen dar, die durch klonale Akkumulation von T-Lymphozyten-Populationen charakterisiert sind. Die Diagnose wird in der Regel aufgrund klinischer und histologischer charakteristischer Eigenschaften der CTCL gestellt. Schwierigkeiten bereitet die Diagnosestellung insbesondere in frühen Stadien, aber auch in seltenen Varianten der CTCL. In diesen Fällen ist der Nachweis klonaler T-Zell-Populationen durch identisch umgelagerte T-Zellrezeptor-Gene eine wertvolle Hilfe. So zeigen PCR-basierende Techniken zum Nachweis klonaler T-Zell-Populationen eine hohe Sensitivität von 70 - 90 % in Hautbiopsien früher CTCL-Stadien. Nach Diagnosestellung ist der nächste wichtige Schritt die Ausbreitungsdiagnostik des CTCL, sinkt doch die Überlebensrate drastisch bei Auftreten extrakutaner Manifestationen wie z.B. bei Infiltration der Lymphknoten oder viszeraler Organe. Ein Stadienwechsel, d.h. die Progression der Erkrankung, wird in der Regel klinisch erst spät entdeckt. Insbesondere der Lymphknotenbefall, der erfahrungsgemäß als erste extrakutane Manifestation des CTCL gilt, stellt sowohl den Kliniker als auch den Histologen vor diagnostische Probleme. Hier konnten wir in einer aktuellen Studien zeigen, dass in ca. 50 % der dermatopathischen Lymphknoten identische klonale T-Zell-Populationen wie in den CTCL-Läsionen der Haut nachweisbar sind. Des Weiteren zeigte sich, dass diese Patienten eine reduzierte Überlebensrate haben, ähnlich denen, die eine histologisch manifeste Lymphknoteninfiltration durch das CTCL haben. Diese Daten belegen, dass T-Zellrezeptor-Umlagerungsanalysen bei Patienten mit CTCL sowohl eine diagnostische als auch prognostische Bedeutung haben und somit ein akkurates Staging ermöglichen.
Abstract
Cutaneous T-cell lymphoma (CTCL) is a clonal lymphoproliferative malignancy primarily involving the skin. Routine diagnosis of CTCL is based on its characteristic clinical and histopathologic features. However, the broad clinical and histological spectrum of the disease, especially of its early stages and rare variants, often complicates the differentiation between malignant CTCL and benign lymphoproliferative or reactive skin diseases. In these cases demonstration of a clonal T-cell population by detection of identically rearranged T-cell receptor genes (TCR) provides an adjunct. Using this approach clonality could be demonstrated in 70-90 % in CTCL biopsies of early stages by recent PCR-based studies, indicating a high sensitivity. After achieving the correct diagnosis the next important step is to stage the disease in CTCL-patients. Patients with CTCL usually have an indolent course with a 5-year survival of approximately 87%. However, in a significant number of patients the disease may progress and disseminated extracutaneous manifestations may develop involving the lymph nodes, blood and visceral organs. The prognosis for patients with widespread manifestation of CTCL beyond the skin is poor. Therefore, accurate evaluation in each individual case is crucial for an adequate, stage-adapted therapeutic approach. Determining the status of peripheral lymph nodes, generally the first site of extracutaneous dissemination is particularly important for clinical staging of patients with CTCL. Recently we could demonstrate in a large cohort of CTCL patients that nearly 50 % of dermatopathic lymph nodes of CTCL patients harbor a clonal T-cell population. Moreover, clonal T-cell detection in dermatopathic lymph nodes of CTCL patients was associated with limited survival similar to patients with histologically confirmed lymph node involvement, whereas all patients without T-cell clonality in the lymph nodes were alive at the last follow-up. In conclusion, these results suggest that TCR rearrangement analysis has an important impact in the initial diagnosis of CTCL and, moreover PCR analysis of lymph nodes is an important additional step in achieving an accurate clinical staging.
Literatur
-
1
Diamandidou E, Cohen P R, Kurzrock R.
Mycosis fungoides and Sézary syndrome.
Blood.
1996;
88
2385-409
-
2
Balfour E M, Glusac E J, Heald P, Talley L L, Smoller B R.
Sezary syndrome: cutaneous immunoperoxidase double-labeling technique demonstrates CD4/CD8 ratio non-specificity.
J Cutan Pathol.
2003;
30
437-442
-
3
Hoppe R T, Wood G S, Abel E A.
Mycosis fungoides and the Sézary syndrome: pathology, staging, and treatment.
Curr Probl Cancer.
1990;
14
293-371
-
4
Diamandidou E, Cohen P R, Kurzrock R.
Mycosis fungoides and Sézary syndrome.
Blood.
1996;
88
2385-409
-
5
Zackheim H S, Amin S, Kashani-Sabet M, McMillan A.
Prognosis in cutaneous T-cell lymphoma by skin stage: long-term survival in 489 patients.
J Am Acad Dermatol.
1999;
40
418-25
-
6
Diamandidou E, Colome M, Fayad L, Duvic M, Kurzrock R.
Prognostic factor analysis in mycosis fungoides/Sézary syndrome.
J Am Acad Dermatol.
1999;
40
914-924
-
7
De Coninck E C, Kim Y H, Varghese A, Hoppe T R.
Clinical characteristics and outcome of patients with extracutaneous mycosis fungoides.
J Clin Oncol.
2001;
19
779-784
-
8
Kohler S, Kim Y H, Smoller B R.
Histologic criteria for the diagnosis of erythrodermic mycosis fungoides and Sezary syndrome: a critical reappraisal.
J Cutan Pathol.
1997;
24
292-297
-
9
Smoller B R.
.
Pathol Case Rev.
1996;
1
158-162
-
10
Trotter M J, Whittaker S J, Orchard G E, Smith N P.
Cutaneous histopathology of Sézary syndrome: a study of 41 cases with a proven circulating T-cell clone.
J Cutan Pathol.
1997;
24
286-291
-
11
Glusac E J.
Criterion by criterion, mycosis fungoides.
Am J Dermatopathol.
2003;
25
264-269
-
12
Olerud J E, Kulin P A, Chew D E, Carlsen R A, Hammar S P, Weir T W, Patterson S D, Bolen J W, Kadin M E, Barker E. et al .
Cutaneous T-cell lymphoma. Evaluation of pretreatment skin biopsy specimens by a panel of pathologists.
Arch Dermatol.
1992;
128
501-507
-
13
Alt F W, Oltz E M, Young F, Gorman J, Taccioli G, Chen J.
VDJ recombination.
Immunol Today.
1992;
13
306-314
-
14
Bourguin A, Tung R, Galili N, Sklar J.
Rapid, nonradioactive detection of clonal T-cell receptor gene rearrangements in lymphoid neoplasms.
Proc Natl Acad Sci USA.
1990;
87
8536-8540
-
15
Hodges E, Krishna M T, Pickard C, Smith J L.
Diagnostic role of tests for T-cell receptor (TCR) genes.
J Clin Pathol.
2003;
56
1-11
-
16
Bottaro M, Berti E, Biondi A, Migone N, Crosti L.
Heteroduplex analysis of T-cell receptor gamma gene rearrangements for diagnosis and monitoring of cutaneous T-cell lymphomas.
Blood.
1994;
83
3271-3278
-
17
Wood G S, Tung R M, Haeffner A C, Crooks C F, Liao S, Orozco R, Veelken H, Kadin M E, Koh H, Heald P, Barnhill R L, Sklar J.
Detection of clonal T-cell receptor gamma gene rearrangements in early Mycosis fungoides/sézary syndrome by polymerase chain reaction and denaturing gradient gel electrophoresis (PCR/DGGE).
J Invest Dermatol.
1994;
103
34-41
-
18
Theodorou I, Delfau L arue, Bigorgne C, Lahet C, Cochet G, Bagot M, Wechsler J, Farcet J P.
Cutaneous T-cell infiltrates: Analysis of T-cell receptor gamma gene rearrangement by polymerase chain reaction and denaturing gradient gel electrophoresis.
Blood.
1995;
86
305-310
-
19
Dippel E, Assaf C, Hummel M, Schrag H J, Stein H, Goerdt S, Orfanos C E.
Clonal T-cell receptor gamma-chain gene rearrangement by PCR-based GeneScan analysis in advanced cutaneous T-cell lymphoma: a critical evaluation.
J Pathol.
1999;
188
46-154
-
20
Assaf C, Hummel M, Dippel E, Goerdt S, Muller H H, Anagnostopoulos I, Orfanos C E, Stein H.
High detection rate of T-cell receptor beta chain rearrangements in T-cell lymphoproliferations by family specific polymerase chain reaction in combination with the GeneScan technique and DNA sequencing.
Blood.
2000;
96
640-646
-
21
Yoshikai Y, Toyonaga B, Yasuhiro K, Kimura N, Griesser H, Mak T W.
Repertoire of the human T-cell gamma genes: High frequency of nonfunctional transcripts in thymus and mature T cells.
Eur J Immunol.
1987;
17
119-126
-
22
Harris N L, Stein H, Coupland S E, Hummel M, Favera R D, Pasqualucci L, Chan W C.
New approaches to lymphoma diagnosis.
Hematology (Am Soc Hematol Educ Program).
2001;
194-220
-
23
Scheller U, Muche J M, Sterry W, Lukowsky A.
Detection of clonal T cells in cutaneous T cell lymphoma by polymerase chain reaction: comparison of mutation detection enhancement-polyacrylamide gel electrophoresis, temperature gradient gel electrophoresis and fragment analysis of sequencing gels.
Electrophoresis.
1998;
19
653-658
-
24
Assaf C, Hummel M, Dippel E. et al .
Common clonal T-cell origin in patient with T-prolymphocytic leukemia and associated cutaneous T-cell lymphomas.
Br J Hematol.
2003;
120
488-491
-
25
Assaf C, Hummel M, Zemlin M, Steinhoff M, Geilen C C, Stein H, Orfanos C E.
Transition of Sezary syndrome into mycosis fungoides after complete clinal and molecular remission under extracorporeal photophoresis.
J Clin Pathol.
2004;
57
1325-1328
-
26
Klemke C D, Dippel E, Dembinski A, Ponitz N, Assaf C, Hummel M, Stein H, Goerdt S.
Clonal T cell receptor gamma-chain gene rearrangement by PCR-based GeneScan analysis in the skin and blood of patients with parapsoriasis and early-stage mycosis fungoides.
J Pathol.
2002;
197
348-354
-
27
Bergman R.
How useful are T-cell receptor gene rearrangement studies as an adjunct to the histopathologic diagnosis of mycosis fungoides?.
Am J Dermatopathol.
1999;
21
498-502
-
28
Burg G, Dummer R, Haeffner A, Kempf W, Kadin M.
From inflammation to neoplasia: mycosis fungoides evolves from reactive inflammatory conditions (lymphoid infiltrates) transforming into neoplastic plaques and tumors.
Arch Dermatol.
2001;
137
949-952
-
29
Cherny S, Mraz S, Su L, Harvell J, Kohler S.
Heteroduplex analysis of T-cell receptor gamma gene rearrangement as an adjuvant diagnostic tool in skin biopsies for erythroderma.
J Cutan Pathol.
2001;
28
351-355
-
30
Cordel N, Lenormand B, Courville P, Lauret P, Joly P.
Detection of clonal T-cell receptor gamma gene rearrangement with the use of PCR-DGGE for diagnosis of erythroderma.
Ann Dermatol Venereol.
2001;
128
220-223
-
31
Geissmann F, Dieu-Nosjean M C, Dezutter C, Valladeau J, Kayal S, Leborgne M, Brousse N, Saeland S, Davoust J.
Accumulation of immature Langerhans cells in human lymph nodes draining chronically inflamed skin.
J Exp Med.
2002;
196
417-430
-
32
Rausch E, Kaiserling E, Goos M.
Langerhans cells and interdigitating reticulum cells in the thymus-dependent region in human dermatopathic lymphadenitis.
Virchows Arch B Cell Pathol.
1977;
25
327-343
-
33
van der Valk P, Meijer C J.
The histology of reactive lymph nodes.
Am J Surg Pathol.
1987;
11
866-882
-
34
Weiss L M, Wood G S, Warnke R A.
Immunophenotypic differences between dermatopathic lymphadenopathy and lymph node involvement in mycosis fungoides.
Am J Pathol.
1985;
120
179-185
-
35
Weiss L M, Hu E, Wood G S, Moulds C, Cleary M L, Warnke R, Sklar J.
Clonal rearrangements of T-cell receptor genes in mycosis fungoides and dermatopathic lymphadenopathy.
N Engl J Med.
1985;
313
539-544
-
36
Lynch J W Jr., Linoilla I, Sausville E A, Steinberg S M, Ghosh B C, Nguyen D T, Schechter G P, Fischmann AB, Ihde D C, Stocker J L, Bastian A, Turner R, Cotelingam J D, Gazdar A F, Foss F M.
Prognostic implications of evaluation for lymph node involvement by T-cell antigen receptor gene rearrangement in mycosis fungoides.
Blood.
1992;
79
3293-3299
-
37
Bakels V, Van Oostveen J W, Geerts ML, Gordijn R L, Walboomers J M, Scheffer E, Meijer C J, Willemze R.
Diagnostic and prognostic significance of clonal T-cell receptor beta gene rearrangements in lymph nodes of patients with mycosis fungoides.
J Pathol.
1993;
170
249-255
-
38
Kern D E, Kidd P G, Moe R, Hanke D, Olerud J E.
Analysis of T-cell receptor gene rearrangement in lymph nodes of patients with mycosis fungoides. Prognostic implications.
Arch Dermatol.
1998;
134
158-164
-
39
Assaf C, Hummel M, Steinhoff M, Geilen C C, Orawa H, Stein H, Orfanos C E.
Early TCR-β and TCR-γ PCR detection of clonality indictes minimal tumor disease in lymph nodes of cutaneous T-cell lymphoma: diagnostic and prognostic implications.
Blood.
2004: Online-Publication 30. 9. 2004 ;
-
40
Vonderheid E C, Sobel E L, Nowell P C, Finan J B, Helfrich M K, Whipple D S.
Diagnostic and prognostic significance of Sezary cells in peripheral blood smears from patients with cutaneous T cell lymphoma.
Blood.
1985;
66
358-366
-
41
Sterry W, Mielke V.
CD4+ cutaneous T-cell lymphomas show the phenotype of helper/inducer T cells (CD45RA-, CDw29+).
J Invest Dermatol.
1989;
93
413-416
-
42
Wood G S, Hong S R, Sasaki D T, Abel E A, Hoppe R T, Warnke R A, Morhenn V B.
Leu-8/CD7 antigen expression by CD3+ T cells: comparative analysis of skin and blood in mycosis fungoides/Sezary syndrome relative to normal blood values.
J Am Acad Dermatol.
1990;
22
602-607
-
43
Bernengo M G, Quaglino P, Novelli M, Cappello N, Doveil G C, Lisa F, de Matteis A, Fierro M T, Appino A.
Prognostic factors in Sezary syndrome: a multivariate analysis of clinical, haematological and immunological features.
Ann Oncol.
1998;
9
857-863
-
44
Payne C M, Glasser L.
Ultrastructural morphometry in the diagnosis of Sezary syndrome.
Arch Pathol Lab Med.
1990;
114
661-671
-
45
Preesman A H, Schrooyen S J, Toonstra J, van der Putte S C, Rademakers L H, Willemze R, van Vloten W A.
The diagnostic value of morphometry on blood lymphocytes in erythrodermic actinic reticuloid.
Arch Dermatol.
1995;
131
1298-1303
-
46
Gorochov G, Bachelez H, Cayuela J M, Legac E, Laroche L, Dubertret L, Sigaux F.
Expression of V beta gene segments by Sezary cells.
J Invest Dermatol.
1995;
105
56-61
-
47
Bigler R D, Boselli C M, Foley B, Vonderheid E C.
Failure of anti-T-cell receptor V beta antibodies to consistently identify a malignant T-cell clone in Sezary syndrome.
Am J Pathol.
1996;
149
1477-1483
-
48
Limon J, Nedoszytko B, Brozek I, Hellmann A, Zajaczek S, Lubinski J, Mrozek K.
Chromosome aberrations, spontaneous SCE, and growth kinetics in PHA-stimulated lymphocytes of five cases with Sezary syndrome.
Cancer Genet Cytogenet.
1995;
83
75-81
-
49
Thangavelu M, Finn W G, Yelavarthi K K, Roenigk H H, Jr., Samuelson E, Peterson L, Kuzel T M, Rosen S T.
Recurring structural chromosome abnormalities in peripheral blood lymphocytes of patients with mycosis fungoides/Sézary syndrome.
Blood.
1997;
89
3371-3377
-
50
Weiss L M, Wood G S, Hu E, Abel E A, Hoppe R T, Sklar J.
Detection of clonal T-cell receptor gene rearrangements in the peripheral blood of patients with mycosis fungoides/Sezary syndrome.
J Invest Dermatol.
1989;
92
601-604
-
51
Bakels V, van Oostveen J W, Gordijn R L, Walboomers J M, Meijer C J, Willemze R.
Diagnostic value of T-cell receptor beta gene rearrangement analysis on peripheral blood lymphocytes of patients with erythroderma.
J Invest Dermatol.
1991;
97
782-786
-
52
Vonderheid E C, Sobel E L, Nowell P C, Finan J B, Helfrich M K, Whipple D S.
Diagnostic and prognostic significance of Sezary cells in peripheral blood smears from patients with cutaneous T cell lymphoma.
Blood.
1985;
66
358-366
-
53
Vonderheid E C, Bernengo M G, Burg G, Duvic M, Heald P, Laroche L, Olsen E, Pittelkow M, Russell-Jones R, Takigawa M, Willemze R.
ISCL Update on erythrodermic cutaneous T-cell lymphoma: report of the International Society for Cutaneous Lymphomas.
J Am Acad Dermatol.
2002;
46
95-106
-
54
Fraser-Andrews E A, Woolford A J, Russell-Jones R, Seed P T, Whittaker S J.
Detection of a peripheral blood T cell clone is an independent prognostic marker in mycosis fungoides.
J Invest Dermatol.
2000;
114
117-121
-
55
Muche J M, Lukowsky A, Asadullah K, Gellrich S, Sterry W.
Demonstration of frequent occurrence of clonal T cells in the peripheral blood of patients with primary cutaneous T-cell lymphoma.
Blood.
1997;
90
1636-1642
-
56
Posnett D N, Sinha R, Kabak S, Russo C.
Clonal populations of T cells in normal elderly humans: the T cell equivalent to ”benign monoclonal gammapathy".
J Exp Med.
199;
179
609-618
-
57
Schwab R, Szabo P, Manavalan J S, Weksler M E, Posnett D N, Pannetier C, Kourilsky P, Even J.
Expanded CD4+ and CD8+ T cell clones in elderly humans.
Immunol.
1997;
158
4493-4499
-
58
Khan N, Shariff N, Cobbold M, Bruton R, Ainsworth J A, Sinclair A J, Nayak L, Moss P A.
Cytomegalovirus seropositivity drives the CD8 T cell repertoire toward greater clonality in healthy elderly individuals.
J Immunol.
2002;
169
1984-1992
-
59
Muche J M, Sterry W, Gellrich S, Rzany B, Audring H, Lukowsky A.
Peripheral blood T-cell clonality in mycosis fungoides and nonlymphoma controls.
Diagn Mol Pathol.
2003;
12
142-150
-
60
Delfau-Larue M H, Laroche L, Wechsler J, Lepage E, Lahet C, Asso M, Bagot M, Farcet J P.
Diagnostic value of dominant T-cell clones in peripheral blood in 363 patients presenting consecutively with a clinical suspicion of cutaneous lymphoma.
Blood.
2000;
96
2987-2992
-
61
Beylot-Barry M, Sibaud V, Thiebaut R, Vergier B, Beylot C, Delaunay M, Chene G, Dubus P, Merlio J P.
Evidence that an identical T cell clone in skin and peripheral blood lymphocytes is an independent prognostic factor in primary cutaneous T cell lymphomas.
J Invest Dermatol.
2001;
117
920-926
-
62
van Dongen J J, Langerak A W, Bruggemann M, Evans P A, Hummel M, Lavender F L, Delabesse E, Davi F, Schuuring E, Garcia-Sanz R, van Krieken J H, Droese J, Gonzalez D, Bastard C, White H E, Spaargaren M, Gonzalez M, Parreira A, Smith J L, Morgan G J, Kneba M, Macintyre E A.
Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98 - 3936.
Leukemia.
2003;
17
2257-317
Dr. med. Chalid Assaf
Klinik und Hochschulambulanz für Dermatologie, Charité - Campus Benjamin Franklin
Fabeckstraße 60 - 62 · 14195 Berlin
eMail: chalid.assaf@charite.de