Moderate Exercise with a Dietary Vitamin C and E Combination Protects Against Streptozotocin-Induced Oxidative Damage to the Kidney and Lens in Pregnant Rats

Abstract

Moderate exercise and vitamin C and E (VCE) supplementation can be beneficial to diabetes due to reducing free radical production in lens and kidney of diabetic pregnant rats. We investigated the effect of VCE supplementation and moderate exercise on lipid peroxidation (MDA) and scavenging enzyme activity in the kidneys and lens of STZ-induced diabetic pregnant rats.

Fifty female Wistar rats were used and were randomly divided into five groups. First and second were used as the control and pregnant control group. Third group was the pregnant diabetic group. The fourth group was the diabetic-pregnant-exercise group. VCE-supplemented feed was given to pregnant-diabetic-exercise rats constituting the fifth group. Animals in the exercised groups were moderately exercised daily on a treadmill (16.1 m/min, 45 min/d) for three weeks (five days a week). Diabetes was induced on day zero of the study. Plasma, lens, and kidney samples were taken from all animals on day 20.

Exercise and administration of VCE to pregnant diabetic rats resulted in significant decrease in the albumin and total protein values and the elevated MDA, plasma creatinine, and urea levels as an indicator of oxidative stress and renal functional parameters. Exercise and VCE supplementation also increased glutathione peroxidase (GSH-Px), reduced glutathione (GSH), vitamin E, and beta-carotene levels in the kidney, GSH-Px and GSH in the lens, the albumin and total protein values in plasma. In the diabetic pregnant animals, the decreased vitamins A and E concentration and GSH levels in kidney, creatinine, and urea values in plasma did not improve through exercise only although their concentrations were increased by VCE supplementation. Kidney weight did not also affect either by exercise or VCE supplementation.

In conclusion, these results suggest that exercise plus VCE affects antioxidant metabolism and reduces lipid peroxidation, thereby improving the damage caused by oxidative stress involved in the pathogenesis of lens and kidney in diabetic pregnant rats. Moderate exercise with dietary VCE may play a role in preventing nephropathy and cataract formation in diabetic pregnant rat.

Key words

Oxidative stress - diabetes - exercise - pregnancy - vitamin C and E

References

• 1 Akova B, Sürmen-Gür E, Gür H, Dirican M, Sarandöl E, Kücükoglu S. Exercise induced oxidative stress and muscle performance in healthy women: role of vitamin E supplementation and endogenous oestradiol.  Eur J Appl Physiol. 2001;  84 141-147
• 2 Atalay M, Laaksonen D E. Diabetes, oxidative stress and physical exercise.  J Sports Sci Med. 2002;  1 1-14
• 3 Czernichow S, Hercberg S. Interventional studies concerning the role of antioxidant vitamins in cardiovascular diseases: A review.  J Nutr Health Aging. 2001;  5 188-195
• 4 Damasceno D C, Volpato G T, Calderon I MP, Rudge M VC. Oxidative stress and diabetes in pregnant rats.  Anim Rep Sci. 2002;  72 235-244
• 5 Desai I D. Vitamin E analysis methods for animal tissues.  Methods Enzymol. 1984;  105 138-147
• 6 Fulop T, Larbi A, Douziech N. Insulin receptor and ageing.  Pathol Biol. 2003;  51 574-580
• 7 Gul M, Laaksonen D E, Atalay M, Vider L, Hanninen O. Effects of endurance training on tissue glutathione homeostasis and lipid peroxidation in streptozotocin-induced diabetic rats.  Scand J Med Sports. 2002;  12 163-170
• 8 Ji L L, Stratman F W, Lardy H A. Enzymatic down regulation with exercise in rat skeletal muscle.  Arch Biochem Biophys. 1988;  263 139-149
• 9 Kedziora-Kornatowska K, Szram S, Kornatowski T, Szadujkis-Szadurski L, Kedziora J, Bartosz G. Effects of vitamin E and vitamin C supplementation on antioxidantive state and renal glomerular basement membrane thickness in diabetic kidney.  Nephron Exp Nephrol. 2003;  95 134-143
• 10 Kinalski M, Sledziewski A, Telejko B, Zarzycki W, Kinalska I. Antioxidant therapy and streptozotocin-induced diabetes in pregnant rats.  Acta Diabetol. 1999;  36 113-117
• 11 Kriskal B S, Vigneau-Callahan K E, Moskowitz A J, Matson W R. Purine catabolism: links to mitochondrial respiration and antioxidant defenses?.  Arch Biochem Biophys. 1999;  370 22-33
• 12 Kutlu M, Nazıroğlu M, Yılmaz T, Kükner A Ş. Moderate exercise with a dietary vitamins C and E combination counteracts oxidative stress in kidney and lens of streptozotocin-induced diabetic rat.  Int J Vitam Nutr Res. 2004;  74 (in press)
• 13 Lawrence R A, Burk R F. Glutathione peroxidase activity in selenium-deficient rat liver.  Biochem Biophys Res Commun. 1976;  71 952-958
• 14 Lee D M, Hoffman W H, Carl G F, Khichi M, Cornwell P E. Lipid peroxidation and antioxidant vitamins prior to, during, and after correction of diabetic ketoacidosis.  J Diab Comp. 2002;  16 294-300
• 15 Lin J. Pathophysiology of cataracts: copper ion and peroxidation in diabetics.  Jpn J Ophthalmol. 1997;  41 130-137
• 16 Lowry O H, Rosebrough N J, Farr A L, Randall R J. Protein measurement with the Folin-Phenol reagent.  J Biol Chem. 1951;  193 265-275
• 17 Nazıroğlu M, Çay M. Protective role of intraperitoneally administered vitamin E and selenium on the antioxidative defense mechanism in rats with diabetes induced streptozotocin.  Biol Trace Elem Res. 2001;  79 149-159
• 18 Nazıroğlu M, Şimşek M, Kutlu M. Moderate exercise with dietary vitamin C and E combination protects streptozotocin-induced oxidative damage to the blood and improves fetal outcomes in pregnant rats.  Clin Chem Lab Med. 2004 a;  42 511-517
• 19 Nazıroğlu M, Şimşek M, Şimşek H, Aydilek N, Özcan Z, Atılgan R. Effects of hormone replacement therapy, vitamin C and E supplementation on antioxidants levels, lipid profiles and glucose homeostasis in postmenopausal women with Type 2.  Clin Chim Acta. 2004 b;  344 63-71
• 20 Nazıroğlu M. Enhanced testicular antioxidant capacity in streptozotocin induced diabetic rats: Protective role of vitamins C, E and selenium.  Biol Trace Elem Res. 2003;  94 61-71
• 21 Packer L, Landvik S. Vitamin E in biological systems. Emerit I Packer L, Auclair C Antioxidants in Therapy and Preventive Medicine. New York; Plenum Press 1990: 93-104
• 22 Placer Z A, Cushman L, Johnson B C. Estimation of products of lipid peroxidation (malonyl dialdehyde) in biological fluids.  Anal Biochem. 1966;  16 359-364
• 23 Radak Z, Kaneko T, Tahara S, Nakamoto H, Ohno H, Sasvari M, Nyakas C, Goto S. The effects of exercise training on oxidative damage of lipids, proteins, and DNA in rat skeletal muscle: Evidence for beneficial outcomes.  Free Radic Biol Med. 1999;  27 69-74
• 24 Sedlak J, Lindsay R HC. Estimation of total, protein bound and non-protein sulfhydryl groups in tissue with Ellmann' s reagent.  Anal Biochem. 1968;  25 192-205
• 25 Siman C M, Eriksson U J. Vitamin E decreases the occurrence of malformations in the offspring of diabetic rats.  Diabetes. 1997 a;  46 1054-1061
• 26 Siman C M, Eriksson U J. Vitamin C supplementation of the maternal diet reduces the rate of malformation in the offspring of diabetic rats.  Diabetologia. 1997 b;  40 416-424
• 27 Suzuki J, Katoh N. A simple and cheap method for measuring vitamin A in cattle using only a spectrophotometer.  Jpn J Vet Sci. 1990;  52 1282-1284
• 28 Uriu-Hare J Y, Keen C L, Applegate E A, Stern J S. The influence of moderate exercise in diabetic and normal pregnancy on maternal and fetal outcome in the rat.  Life Sci. 1989;  45 647-654
• 29 Yadav P, Sarkars S, Bhatnagar D. Action of capparis deciduas against alloxan-induced oxidative stress and diabetes in rat tissues.  Pharmacol Res. 1997;  36 221-228