Planta Med 2004; 70(10): 986-992
DOI: 10.1055/s-2004-832626
Original Paper
Analytical Methods
© Georg Thieme Verlag KG Stuttgart · New York

Generation and Characterisation of Monoclonal Antibodies to Oleanolic Acid

K. M. G. Brand1 , C. Klein2 , I. Zündorf2 , T. Dingermann2 , W. Knöss1
  • 1Institute of Pharmaceutical Biology, Rheinische Friedrich-Wilhelms-Universität Bonn, Bonn, Germany
  • 2Institute of Pharmaceutical Biology, Johann Wolfgang-Goethe-Universität Frankfurt, Frankfurt, Germany
Further Information

Publication History

Received: March 2, 2004

Accepted: July 15, 2004

Publication Date:
18 October 2004 (online)

Abstract

Monoclonal antibodies to oleanolic acid, a pentacyclic triterpene, were generated using the hybridoma fusion method described by Köhler and Milstein [1]. Protein conjugates of the target molecule for immunisation were prepared either by directly linking the isoprenoid to different protein carriers via the carboxylic function or after introduction of a succinic spacer between the protein carrier and position 3 of the target molecule. Antibodies of three different cell lines were further analysed by competitive ELISA and were shown to be directed either against both rings A and B or to ring E of the pentacyclic system. Thus these antibodies can be used in the specific detection of oleanolic acid itself or of a broad range of oleanolic acid derivatives sharing a specific structural moiety. Therefore these antibodies may be interesting tools for the screening of putative terpenoid-containing plants.

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Prof. Dr. Theodor Dingermann

Institut für Pharmazeutische Biologie

Johann Wolfgang Goethe-Universität

Marie-Curie-Str. 9

60439 Frankfurt

Germany

Fax: +49-69-7982-9662

Email: dingermann@em.uni-frankfurt.de