Planta Med 2004; 70(10): 1003-1005
DOI: 10.1055/s-2004-832629
Letter
© Georg Thieme Verlag KG Stuttgart · New York

Red Clover Extracts Stimulate Differentiation of Human Osteoblastic Osteosarcoma HOS58 Cells

K. Wende1 , L. Krenn2 , I. Unterrieder2 , U. Lindequist1
  • 1Institute of Pharmacy, University of Greifswald, Greifswald, Germany
  • 2Institute of Pharmacognosy, University of Vienna, Vienna, Austria
Further Information

Publication History

Received: April 27, 2004

Accepted: June 25, 2004

Publication Date:
18 October 2004 (online)

Abstract

Different extracts from red clover (Trifolium pratense L.) were tested for their ability to stimulate the activity of osteoblastic osteosarcoma cells (HOS58). As a key marker of osteoblasticity we chose the activity of alkaline phosphatase (ALP). Whereas butanol and methanol extracts had no influence on either ALP or cellular protein production, enzyme activity was increased significantly on incubation with chloroform extracts. All extracts were analysed for isoflavone content. The data clearly suggest a role for red clover isoflavonoids in the stimulation of osteoblastic cell activity.

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L. Krenn

Institute of Pharmacognosy

University of Vienna

Althanstraße 14

1090 Vienna

Austria

Fax: +43-1-4277-9552

Email: liselotte.krenn@univie.ac.at