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Planta Med 2004; 70(11): 1022-1026
DOI: 10.1055/s-2004-832641
Original Paper
Pharmacology
© Georg Thieme Verlag KG Stuttgart · New York

Casuarinin Protects Cultured MDCK Cells from Hydrogen Peroxide-Induced Oxidative Stress and DNA Oxidative Damage

Ching-Hsein Chen1 , 7 , Tsan-Zon Liu2 , 7 , Tsun-Cheng Kuo3 , Fung-Jou Lu4 , Yu-Chin Chen5 , Yi-Wen Chang-Chien1 , Chun-Ching Lin6
  • 1Department of Medical Technology, Fooyin University, Ta-Liao, Kaohsiung Hsien, Taiwan, ROC
  • 2Center for Gerontological Research and Graduate Institute of Medical Biotechnology, Chang Gung University, Taoyuan, Taiwan, ROC
  • 3Department of Cosmetic Science, Chia-Nan University of Pharmacy and Science Tainan, Taiwan, ROC
  • 4Department of Applied Chemistry, Chung Shan Medical University, Taichung, Taiwan, ROC
  • 5Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan, ROC
  • 6Graduate Institute of Pharmaceutical Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC
  • 7These authors contributed equally to this work
Further Information

Publication History

Received: February 17, 2004

Accepted: June 26, 2004

Publication Date:
18 November 2004 (online)

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Abstract

Casuarinin has been shown to be an antioxidant in acellular experiments. This study was designed to assess the ability of casuarinin, extracted from Terminalia arjuna, to protect cultured Madin-Darby canine kidney (MDCK) cells against H2O2-mediated oxidative stress. A comparison with trolox, a hydrosoluble vitamin E analogue was performed. MDCK cells were pretreated with casuarinin or trolox for 1 h, then exposed to H2O2. After incubation with 0.8 mM H2O2 for 1 h, casuarinin caused a decrease in intracellular peroxide production as shown by dichlorofluorescein (DCF) fluorescence in a concentration-dependent manner. After 3 h exposure to 8 mM H2O2, the percentage of intracellular glutathione (GSH)-negative cells was reduced in the casuarinin-treated group. Addition of 32mM H2O2 to MDCK cells for 3 h induced an increase in the percentage of cells containing 8-oxoguanine but the level of such cells declined in casuarinin-treated cells. These results show that casuarinin is more effective against H2O2-induced oxidative damage than trolox. The data suggest that casuarinin attenuates H2O2-induced oxidative stress, decreases DNA oxidative damage and prevents the depletion of intracellular GSH in MDCK cells.

Key words

Casuarinin - Terminalia arjuna - oxidative stress - hydrogen peroxide - glutathione - DNA oxidative damage

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Professor Chun-Ching Lin

Graduate Institute of Pharmaceutical Science

College of Pharmacy

Kaohsiung Medical University

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