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DOI: 10.1055/s-2004-832645
© Georg Thieme Verlag KG Stuttgart · New York
Polyacetylenic Compounds and Butanol Fraction from Bidens pilosa can Modulate the Differentiation of Helper T Cells and Prevent Autoimmune Diabetes in Non-Obese Diabetic Mice
Publication History
Received: February 9, 2004
Accepted: April 24, 2004
Publication Date:
18 November 2004 (online)
Abstract
Compelling evidence suggests that infiltrating CD4+ type I helper T (Th1) cells in the pancreatic islets play a pivotal role in the progression of diabetes in non-obese diabetic (NOD) mice. We demonstrate in the present report that a butanol fraction of B. pilosa suppressed the development of diabetes, helped maintain levels of blood sugar and insulin in NOD mice in a dose-dependent manner and elevated the serum IgE levels regulated by Th2 cytokines in NOD mice. Moreover, the butanol fraction inhibited the differentiation of naïve helper T (Th0) cells into Th1 cells but enhanced their transition into type II helper T (Th2) cells using an in vitro T cell differentiation assay. Two polyacetylenic compounds, 2-β-D-glucopyranosyloxy-1-hydroxy-5(E)-tridecene-7,9,11-triyne and 3-β-D-glucopyranosyloxy-1-hydroxy-6(E)-tetradecene-8,10,12-triyne, identified from the butanol fraction also prevented the onset of diabetes like the butanol fraction. The latter compound showed a stronger activity for T cell differentiation than the former. In summary, the butanol fraction of B. pilosa and its polyacetylenes can prevent diabetes plausibly via suppressing the differentiation of Th0 cells into Th1 cells and promoting that of Th0 cells into Th2 cells.
Abbreviations
NOD:non-obese diabetic
IDDM:insulin-dependent diabetes mellitus
PBS:phosphate-buffered saline
PMA:phorbol 12-myristate 13-acetate
PHA:phytohemagglutinin from Phaseolus vulgaris
IFN:interferon
IL:interleukin
LT:lymphotoxin
TNF:tumor necrosis factor
HPLC:high performance liquid chromatography
UV:ultraviolet
FACS:fluorescence activated cell sorter
Th:helper T cells
Th0:naïve Th
Th1:type I Th
Th2:type II Th
i. p.:intraperitoneal
MTT:methylthiazoletetrazolium
IC50:inhibitory concentration 50 %
CC50:cytotoxic concentration 50 %
Key words
T helper cells - Differentiation - non-obese diabetes - autoimmune diseases - Bidens pilosa - Asteraceae
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Dr Wen-Chin Yang
Institute of BioAgricultural Sciences
Academia Sinica
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