Semin Thromb Hemost 2004; 30(4): 461-471
DOI: 10.1055/s-2004-833481
Copyright © 2004 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New York, NY 10001, USA.

Platelet Coagulation-Protein Interactions

Peter N. Walsh1
  • 1Professor of Medicine, Biochemistry and Thrombosis Research Co-Director, The Sol Sherry Thrombosis Research Center, The Sol Sherry Thrombosis Research Center, Departments of Medicine and Biochemistry, Temple University School of Medicine, Philadelphia, Pennsylvania
Further Information

Publication History

Publication Date:
08 September 2004 (online)

The biochemical mechanisms by which activated platelets participate in exposing receptors for the assembly of enzyme-cofactor-substrate complexes at all stages of the blood coagulation cascade are reviewed. Information derived from studies conducted during the last 30 years supports the concept that the initiation of blood coagulation is triggered by exposure of tissue factor at injury sites, leading to the generation of minute quantities of thrombin (limited by tissue factor pathway inhibitor), sufficient to activate platelets, factors XI, VIII, and V, and trigger the consolidation pathway (i.e., the sequential activation of factors XI, IX, X, and prothrombin on the activated platelet surface), leading to the generation of sufficient thrombin to convert fibrinogen to fibrin and effect hemostasis. Platelets localize coagulation to the hemostatic thrombus and protect coagulation enzymes from inhibition by both plasma and platelet inhibitors (e.g., protease nexin 2), thus preventing disseminated intravascular coagulation.

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  • 129 Wistinghausen B, Reischer A, Oddoux C, Ostrer H, Nardi M, Karpatkin M. Severe factor XI deficiency in an Arab family associated with a novel mutation in exon 11.  Br J Haematol. 1997;  99 575-577
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  • 134 Martincic D, Kravtsov V, Gailani D. Factor XI messenger RNA in human platelets.  Blood. 1999;  94 3397-3404
  • 135 Wong M Y, Gurr J A, Walsh P N. The second epidermal growth factor-like domain of human factor IXa mediates factor IXa binding to platelets and assembly of the factor X activating complex.  Biochemistry. 1999;  38 8948-8960
  • 136 Nesheim M E, Pittman D D, Wang J H, Slonosky D, Giles A R, Kaufman R J. The binding of 35S-labeled recombinant factor VIII to activated and unactivated human platelets.  J Biol Chem. 1988;  263 16467-16470
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  • 140 Ahmad S S, Walsh P N. Lipid raft association of the factor VIII C2 domain mediates platelet membrane assembly of factor VIIIa into the factor-X-activating complex.  Blood. 2002;  100 126A
  • 141 Mann K G, Jenny R J, Krishnaswamy S. Cofactor proteins in the assembly and expression of blood clotting enzyme complexes.  Annu Rev Biochem. 1988;  57 915-956
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  • 147 Sims P J, Faioni E M, Wiedmer T, Shattil S J. Complement proteins C5b-9 cause release of membrane vesicles from the platelet surface that are enriched in the membrane receptor for coagulation factor Va and express prothrombinase activity.  J Biol Chem. 1988;  263 18205-18212
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  • 151 Thiagarajan P, Tait J F. Collagen-induced exposure of anionic phospholipid in platelets and platelet-derived microparticles.  J Biol Chem. 1991;  266 24302-24307
  • 152 Weiss H J, Lages B. Platelet prothrombinase activity and intracellular calcium responses in patients with storage pool deficiency, glycoprotein IIb-IIIa deficiency, or impaired platelet coagulant activity-a comparison with Scott syndrome.  Blood. 1997;  89 1599-1611

 Professor
Peter N WalshM.D. Ph.D. 

Sol Sherry Thrombosis Research Center, Temple University School of Medicine

3400 North Broad Street

Philadelphia, PA 19140

Email: pnw@temple.edu