Abstract
The purpose of the present study was to determine the effect of treatment with leptin on gonadotrophin secretion and hypothalamic GnRH, excitatory and inhibitory amino acids release, in prepubertal (15 days old) and peripubertal (30 days old) male rats.
Rats of both ages received a single (ip) injection of 30 µg/kg leptin 60 minutes previous to sacrifice. Serum LH was determined, and the hypothalamus dissected and incubated in Earle's medium. GnRH and amino acids release were determined in the media. LH and GnRH were measured by RIA. Amino acids were assessed by HPLC-UV detection.
In the two prepubertal stages, (prepubertal and peripubertal, 15 and 30 days of age respectively) leptin increased plasmatic LH levels (p < 0.01) and hypothalamic GnRH release (p < 0.01). Glutamate (GLU) release showed an increment in leptin-treated rats (p < 0.01) at both ages, while only the 30 days old rats showed an increment of the aspartate (ASP) release. GABA secretion was not modified by leptin treatment.
In conclusion, the results demonstrated that leptin stimulates the LH-GnRH axis during sexual development in male rats, increasing the secretion of both hormones. The hypothalamic excitatory amino acid neurotransmitter system appears to be involved in this change.
Key words
Leptin - GABA - excitatory amino acids - GnRH - LH - male rats
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Prof. Dr. Pablo Scacchi
Departamento de Fisiología
Facultad de Medicina
Universidad de Buenos Aires
Paraguay 2155
1121 Buenos Aires
Argentina
Phone: + 541159509500(2146/7)
Fax: + 54 11 49 62 89 37
Email: scacchi@mail.retina.ar
