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DOI: 10.1055/s-2005-837757
Honokiol Induces Apoptosis via Cytochrome c Release and Caspase Activation in Activated Rat Hepatic Stellate Cells in vitro
Publikationsverlauf
Received: April 26, 2004
Accepted: September 2, 2004
Publikationsdatum:
27. Januar 2005 (online)
Abstract
The therapeutic goal in liver fibrosis is the reversal of fibrosis and the selective clearance of activated hepatic stellate cells (HSCs) by inducing apoptosis. Over the past several years, we have screened for natural products that mediate apoptosis in activated HSCs. Among the candidate compounds, honokiol, isolated from Magnoliae cortex, was found to induce apoptotic death in activated rat HSCs, while there was no cell viability change in hepatocytes, at concentrations of 12.5 - 50 μM. Apoptosis was identified by DNA fragmentation, activation of caspase-3 and -9, and the proteolytic cleavage of poly(ADP-ribose) polymerase, down-regulation of bcl-2 and the release of mitochondrial cytochrome c into the cytoplasm.
Abbreviations
HSCs:hepatic stellate cells
MTT:3-(4,5-dimethylthiazol-1-yl)2,5-diphenyltetrazolium bromide
PARP:poly(ADP-ribose) polymerase
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Prof. Dong Hwan Sohn
Department of Pharmacy
Wonkwang University
Iksan
Jeonbuk 570-749
Republic of Korea
Fax: +82-63-854-6038
eMail: dhsohn@wonkwang.ac.kr