Planta Med 2005; 71(2): 176-180
DOI: 10.1055/s-2005-837787
Original Paper
Analytical Methods
© Georg Thieme Verlag KG Stuttgart · New York

Miniaturization of the Structure Elucidation of Novel Natural Products - Two Trace Antibacterial Acylated Caprylic Alcohol Glycosides from Arctostaphylos pumila

Jin-Feng Hu1 , Hye-Dong Yoo1 , Caroline T. Williams1 , Eliane Garo1 , Peadar A. Cremin1 , Lu Zeng1 , Helene C. Vervoort1 , Chris M. Lee1 , Shane M. Hart1 , Matt G. Goering1 , Mark O’Neil-Johnson1 , Gary R. Eldridge1
  • 1Lead Discovery and Rapid Structure Elucidation Group, Sequoia Sciences, Inc., San Diego, CA, USA
Further Information

Publication History

Received: May 17, 2004

Accepted: August 29, 2004

Publication Date:
24 February 2005 (online)

Abstract

High-throughput isolation, purification and analysis methods applied to natural products libraries from plants gave rise to the discovery of two novel acylated caprylic alcohol glycosides (1, 2) produced by Arctostaphylos pumila. The NMR spectra were acquired using the CapNMR™ probe and performed on mass-limited samples, which enabled us to elucidate the structures of 2,6-diacetyl-3,4-diisobutyl-1-O-octylglucopyranoside (1, 200 μg) and 2,6-diacetyl-3,4-dimethylbutyl-1-O-octylglucopyranosid (2, 70 μg). Compounds 1 and 2 exhibited antibacterial activity against Gram-positive methicillin-resistant Staphylococcus aureus with an MIC of 128 μg/mL and 64 μg/mL, respectively.

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Dr. Jin-Feng Hu

Lead Discovery & Rapid Structure Elucidation Group

Sequoia Sciences, Inc.

11199 Sorrento Valley Road, Suite H

San Diego

CA 92121

USA

Fax: +1-858-623-0805

Email: jhu@sequoiasciences.com