Serum nitrite/nitrate (NOx) and malondialdehyde (MDA) levels of 40 male alcoholic patients and 14 healthy male controls were investigated. Severity of alcohol withdrawal in alcoholic patients was evaluated by using the Clinical Institute Withdrawal Assessment for Alcohol (CIWA-Ar) scale just before taking the blood samples. NOx levels in serum samples were determined based on the reduction of nitrate to nitrite by vanadium chloride. MDA levels were also determined spectrophotometrically at 540 nm. The total CIWA-Ar score of alcohol-withdrawn patients was found to be 17.7. NOx and MDA levels were significantly increased in alcoholics during alcohol withdrawal as compared to control subjects. In conclusion, we observed increased serum NOx levels and lipid peroxidation during alcohol withdrawal in alcoholic patients.
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I. Tayfun Uzbay, Ph. D.
Gülhane Military Medical Academy
Faculty of Medicine
Department of Medical Pharmacology
Psychopharmacology Research Unit
Etlik 06018
Ankara
Turkey
Phone: +90 312 304 4764
Fax: +90 312 304 2010
Email: tuzbay@gata.edu.tr
