Abstract
Flavonoids, which are main constituents of herbal medicines, have been reported to inhibit the growth of Helicobacter pylori (HP). Therefore, to evaluate the anti-HP activity of some flavonoids (flavanols, flavones, flavonols and isoflavonoids), their effects on the growth and vacuolation of HP as well as the infective properties of HP against HeLa cells were investigated. Catechins, quercetin and naringenin weakly inhibited the growth of HP, but all tested compounds did not inhibit HP infection into KATO III cells and HP urease activity. Quercetin and naringenin inhibited HP VacA vacuolation in HeLa cells with IC50 values of 0.046 and 0.36 mM, respectively. Quercetin also inhibited procaspase-3 activation to caspase-3 in HeLa cells induced by HP VacA toxin, which may induce cell death via the proteolytic activation of a cascade of caspases. However, quercetin did not affect Bax and Bcl-2 protein levels. Based on these findings, quercetin may improve gastric cell death by inhibiting apoptotic signaling by HP VacA toxin.
Abbreviations
HP:Helicobacter pylori
BSA:bovine serum albumin
ESL:enhanced chemiluminescence
MIC:minimum inhibitory concentration
MTT:methylthiazolyldiphenyl-tetrazolium bromide
PBS:phosphate-buffered saline
VacA:Vacuolating cytotoxin
Key words
Helicobacter pylori
- VacA toxin - procaspase-3 - flavonols - flavones - flavanols - isoflavonoids
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Prof. Dong-Hyun Kim
College of Pharmacy
Kyung Hee University
1 Hoegi
Dongdaemun-ku
Seoul 130-701
Korea
Telefon: +82-2-961-0374
Fax: +82-2-966-3885
eMail: dhkim@khu.ac.kr
