Abstract
The effects of the essential oil of Mentha pulegium L. (EOMP), a plant commonly known as ”pennyroyal” or ”poejo” that is used in folk medicine as an abortifaceant, were assessed on the isolated rat myometrium. Myometrial strips were stimulated with 10 nM oxytocin or 10 μM PGF2α . EOMP (10 - 300 μg/mL) concentration-dependently and reversibly inhibited the amplitude of oscillatory contractions, being approximately 3-fold more active against contractions stimulated by oxytocin than those by PGF2α (IC50 values of 45.7 ± 5.6 μg/mL and 160.9 ± 5.9 μg/mL, respectively), although the maximal inhibitory effect occurred at the same concentration (300 μg/mL) in both cases. This action was shared by pulegone (30 - 300 μM), the principal component of the essential oil (IC50 values of 21.8 ± 2.1 μg/mL and 12.7 ± 4.6 μg/mL, respectively). Nifedipine (30 nM - 30 μM) also abolished agonist-stimulated contractions, and was approximately twice and 12 times as potent as EOMP in inhibiting oxytocin- and prostaglandin F2α (PGF2α )-stimulated contractions, respectively. In conclusion, our results show that the essential oil of the abortifaceant plant Mentha pulegium exerts an inhibitory effect on the contractile activity of the isolated rat myometrium. This oil shares a common effect with the voltage-dependent calcium channel (VDCC) blocker nifedipine, although ostensibly acting via a different mechanism. It thus appears that EOMP and pulegone do not exert direct toxic effects on the myometrium per se that would cause abortion, and other possibilities such as systemic metabolism of plant constituents may rather underlie the abusive use of Mentha pulegium in popular medicine.
Key words
Mentha pulegium
- Lamiaceae - uterus - abortifaceant - contraction - pulegone - essential oil
References
Mentha pulegium L. (Labiatae) em músculo liso uterino de rata. 55ª Reunião Anual da SBPC 2003
R )-(+)-pulegone, to a proximate toxin, menthofuran.
Drug Metab Dispos.
1987;
15
589-94
R )-(+)-pulegone in F344 rats.
Drug Metab Dispos.
2001;
29
1567-77
In vivo studies on the metabolism of the monoterpene pulegone in humans using the metabolism of ingestion-correlated amounts (MICA) approach: explanation for the toxicity differences between (S )-(-)- and (R )-(+)-pulegone.
J Agric Food Chem.
2003;
51
6589-97
criddle@liv.ac.uk
