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Semin Reprod Med 2005; 23(1): 38-45
DOI: 10.1055/s-2005-864032
Copyright © 2005 by Thieme Medical Publishers, Inc., 333 Seventh Avenue, New York, NY 10001, USA.

Use of the Progesterone Receptor Antagonist RU 486 to Identify Novel Progesterone Receptor-Regulated Pathways in Implantation

Indrani C. Bagchi1 , Quanxi Li1 , Yong-Pil Cheon1 , Srinivasa Raju Mantena1 , Athilakshmi Kannan1 , Milan K. Bagchi1
  • 1Department of Veterinary Biosciences and Molecular & Integrative Physiology, University of Illinois at Urbana-Champaign, Urbana, Illinois
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Publication History

Publication Date:
15 February 2005 (online)

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ABSTRACT

The steroid hormone progesterone (P) is a critical regulator of embryo implantation and maintenance of pregnancy. P acting through the nuclear progesterone receptors (PRs) regulates the expression of specific gene networks that in turn control the extensive cell proliferation, differentiation, and remodeling that occur in various uterine cell types during the progressive phases of implantation. To identify the P-regulated pathways that underlie the implantation process in the mouse, we employed RU 486, a well-characterized PR antagonist that binds to the receptor and blocks its gene regulatory function. We performed messenger RNA (mRNA) profiling in the peri-implantation uterus using oligonucleotide microarrays to analyze changes in mRNA levels in response to RU 486. This analysis provided, for the first time, a comprehensive profile of PR-regulated gene networks with potential roles during implantation. Our study identified a variety of novel PR-regulated molecules, such as growth factors, protease inhibitors, metabolic enzymes, peptide hormones, transcription factors, immune response molecules, cytoskeletal proteins, and cell adhesion molecules, that are potential mediators of P action in the peri-implantation mouse uterus. This article provides a brief description of the expression and function of these newly identified molecular pathways.

KEYWORDS

Implantation - progesterone - receptor - antiprogestin RU 486

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Indrani C Bagchi

Department of Veterinary Biosciences, University of Illinois at Urbana-Champaign

Urbana, IL 61802

Email: ibagchi@uiuc.edu