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Planta Med 2005; 71(4): 366-369
DOI: 10.1055/s-2005-864106
Letter
© Georg Thieme Verlag KG Stuttgart · New York

In Vitro Antileishmanial Activity of Diphyllin Isolated from Haplophyllum bucharicum

Carole Di Giorgio1 , Florence Delmas1 , Valentina Akhmedjanova3 , Evelyne Ollivier2 , Iraida Bessonova3 , Elias Riad2 , Pierre Timon-David1
  • 1Laboratoire de Parasitologie Hygiène et Zoologie, Faculté de Pharmacie, Marseille, France
  • 2Laboratoire de Pharmacognosie, Faculté de Pharmacie, Marseille, France
  • 3Laboratory of the Chemistry of Alkaloids, S. Yu. Yunusov Institute of the Chemistry of Plant Substances, Tashkent, Uzbekistan
Further Information

Publication History

Received: July 15, 2004

Accepted: December 12, 2004

Publication Date:
27 April 2005 (online)

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Abstract

Diphyllin isolated from Haplophyllum bucharicum Litv. (Rutaceae), an endemic plant of Uzbekistan, displayed a moderate antiproliferative activity towards human monocytes (IC50 = 35.2 μM) and Leishmania promastigotes (IC50 = 14.4 μM), by a mechanism of action that involved interaction with macromolecules and resulted in cell cycle arrest in the S-phase and inhibition of protein synthesis. In the intracellular amastigote form of the parasite, diphyllin exerted a strong specific inhibitory activity (IC50 = 0.2 μM) resulting from the inhibition of parasite internalization within macrophages. This property was mainly due to modulation of macrophage phagocytosis and, to a lesser extent, it also involved interference with surface molecules of the promastigote membrane.

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Carole Di Giorgio

Laboratoire de Parasitologie, Hygiène et Zoologie

Faculté de Pharmacie

27 Bd. Jean Moulin

13385 Marseille Cedex 05

France

Phone: +33-4-91-83-55-44

Fax: +33-4-91-80-26-12

Email: Carole.Digiorgio@Pharmacie.univ-mrs.fr