Planta Med 2005; 71(4): 379-381
DOI: 10.1055/s-2005-864110
Letter
© Georg Thieme Verlag KG Stuttgart · New York

A Xylogalactofucan from the Brown Seaweed Spatoglossum schröederi Stimulates the Synthesis of an Antithrombotic Heparan Sulfate from Endothelial Cells

Hugo Alexandre de Oliveira Rocha1 , 2 , Luana Cristina Lins de Medeiros Bezerra2 , Ivan Rui Lopes de Albuquerque2 , Leandro Silva Costa2 , Celina Maria Pinto Guerra1 , Luiz Diz de Abreu1 , Helena Bonciani Nader3 , Edda Lisboa Leite1 , 2
  • 1Laboratório de Glicobiologia, Departamento de Bioquímica, Universidade Federal de Rio Grande do Norte-UFRN, Natal-RN, Brazil
  • 2Laboratório de Biotecnologia de Polímeros Naturais, Departamento de Bioquímica, Universidade Federal de Rio Grande do Norte-UFRN, Natal-RN, Brazil
  • 3Departamento de Bioquímica, Universidade Federal de São Paulo-UNIFESP, São Paulo-SP, Brazil
Further Information

Publication History

Received: August 13, 2004

Accepted: January 6, 2005

Publication Date:
27 April 2005 (online)

Abstract

The brown seaweed Spatoglossum schröederi (Dictyotaceae) contains three main fucans (fucans A, B and C) with different mobility in electrophoresis. The fucan with highest mobility (fucan C) was precipitated with 2.0 volumes of acetone, purified using a combination of ion exchange chromatography and electrophoresis. It showed an MW of 24 kDa determined by HPLC and Sephadex G-75 chromatography and migrates as a single band in three distinct electrophoretic systems. This fucan contains fucose, xylose, galactose and sulfate in a molar ratio 1 : 0.6 : 2:2.3. The fucan has neither anticoagulant (from 10 to 100 μg) nor hemorrhagic activities (100 μg/mL). In addition, fucan C is neither cytotoxic nor cytostatic. However, fucan C (100 μg/mL) stimulated the synthesis of an antithrombotic heparan sulfate from endothelial cells of rabbit aorta. The results suggest that fucan C might be used as an antithrombotic therapeutic compound.

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Hugo Alexande de Oliveira Rocha

Universidade Federal do Rio Grande do Norte

Centro de Biociências

Departamento de Bioquímica

Laboratório de Biotecnologia de Polímeros Naturais

Av. Salgado Filho S/N

Natal - RN 59097-570

Brazil

Phone: +55-84-211-9208

Fax: +55-84-211-9208

Email: hugo-alexandre@uol.com.br