Abstract
A novel triterpenoid saponin, deapioplatycoside E (1) was isolated from the root extract of Platycodon grandiflorum, together with the seven known saponins 2 - 8, i. e., platycoside E (2), deapioplatycodin D3 (3), platycodin D3 (4), polygalacin D2 (5), platycodin D2 (6), deapioplatycodin D (7) and platycodin D (8). The structure of the new saponin 1 was determined on the basis of spectral analysis and chemical evidence. The crude saponin fraction (ED50: ca. 10 - 15 μg/mL) and compounds 6 - 8 (ED50: ca. 4 - 18 μg/mL) exhibited significant inhibition on the proliferation of five kinds of cultured human tumor cell lines, i. e., A549 (non-small cell lung), SK-OV-3 (ovary), SK-MEL-2 (melanoma), XF498 (central nerve system) and HCT-15 (colon), in vitro.
References
-
1
Lee E B.
Pharmacological studies on Platycodon grandiflorum A. DC. IV. A comparison of experimental pharmacological effects of crude platycodin with clinical indications of Platycodi Radix.
Yakugaku Zasshi.
1973;
93
1188-94
-
2
Takagi K, Lee E B.
Pharmacological studies on Platycodon grandiflorum A. DC.
Activities of crude platycodin on respiratory and circulatory systems and its other pharmacological activities, Yakugaku Zasshi.
1972;
92
969-73
-
3 Lee S H. Method of cultivating the perennial balloon flower. Korean Patent 100 045 791 1991
-
4
Kim K S, Ezaki O, Ikemoto S, Itakura H.
Effects of Platycodon grandiflorum feeding on serum and liver lipid concentrations in rats with diet-induced hyperlipidemia.
J Nutri Sci Vitaminol.
1995;
41
485-91
-
5
Choi C Y, Kim J Y, Kim Y S, Chung Y C, Hahm K S, Jeong H G.
Augmentation of macrophage functions by an aqueous extract isolated from Platycodon grandiflorum
.
Cancer Lett.
2001;
166
17-25
-
6
Choi C Y, Kim J Y, Kim Y S, Chung Y C, Seo J K, Jeong H G.
Aqueous extract isolated from Platycodon grandiflorum elicits the release of nitric oxide and tumor necrosis factor-α from murine macrophages.
Intl Immunopharmacol.
2001;
1
1141-51
-
7
Nikaido T, Koike K, Mitsunaga K, Saeki T.
Two new triterpenoid saponins from Platycodon grandiflorum
.
Chem Pharm Bull.
1999;
47
903-4
-
8
Skehan P, Streng R, Scudiero D, Monks A, McMahon J, Vistica D. et al .
New colorimetric cytotoxicity assay for anticancer-drug screening.
J Natl Cancer Inst.
1990;
82
1107-12
-
9
Ryu S Y, Choi S U, Lee S H, Lee C O, No Z, Ahn J W.
Antitumor triterpenes from medicinal plants.
Arch Pharm Res.
1994;
17
375-7
-
10
Saeki T, Koike K, Nikaido T.
A comparative study on commercial, botanical gardens and wild samples of the roots of Platycodon grandiflorum by HPLC analysis.
Planta Med.
1999;
65
428-31
-
11 Ishii H, Tori K, Tozyo T, Yoshimura Y. Saponins from roots of Platycodon grandiflorum. part 2. Isolation and Structure of new triterpene glycosides. J Chem Soc Perkin Trans 1 1984: 661-8
-
12 Ishii H, Tori K, Tozyo T, Yoshimura Y. Saponins from roots of Platycodon grandiflorum. part 1. structure of prosapogenin. J Chem Soc Perkin Trans 1 1981: 1928-33
Dr. Shi Yong Ryu
Korea Research Institute of Chemical Technology
Yusung
P.O. Box 107
Taejeon 305-343
Korea
Telefon: +82-42-860-7163
Fax: +82-42-860-7160
eMail: syryu@krict.re.kr
