Synthesis and Application of 4′-C-Alkylated Uridines as Probes for Uracil-DNA Glycosylase

Gopinath Rangam; Nicolas Z. Rudinger; H. Martin Müller; Andreas Marx

doi:10.1055/s-2005-865328

PAPER

Synthesis and Application of 4′-C-Alkylated Uridines as Probes for Uracil-DNA Glycosylase

Gopinath Rangam, Nicolas Z. Rudinger, H. Martin Müller, Andreas Marx*
Fachbereich Chemie, Universität Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany
Fax: +49(7531)885140; e-Mail: Andreas.Marx@uni-konstanz.de;

Abstract

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Abstract

The development of new synthetic probes to study the mechanism of Uracil-DNA glycosylase is described. 4′-C-Alkylated 2′-deoxyuridines were synthesized and incorporated site-specifically into oligonucleotides. These compounds were subsequently employed in functional studies of the enzyme and proved to be functional competent.

Key words

DNA - enzymes - glycosylase - nucleotide - oligonucleotides

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References

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