Genetic Markers of Fibrinolytic Responses of Older Persons to Exercise Training

O. Kulaputana; I. Ghiu; D. A. Phares; R. E. Ferrell; R. F. Macko; A. P. Goldberg; J. M. Hagberg

doi:10.1055/s-2005-865813

International Journal of Sports Medicine, Table of Contents

Int J Sports Med 2006; 27(8): 617-622
DOI: 10.1055/s-2005-865813

Training & Testing

Genetic Markers of Fibrinolytic Responses of Older Persons to Exercise Training

O. Kulaputana¹ , I. Ghiu¹ , D. A. Phares¹ , R. E. Ferrell² , R. F. Macko³ , A. P. Goldberg³ , J. M. Hagberg¹

¹Department of Kinesiology, University of Maryland, College Park, MD, USA
²Department of Human Genetics, University of Pittsburgh Graduate School of Public Health, Pittsburgh, PA, USA
³Geriatric Research, Education, and Clinical Center, Baltimore VA Medical Center, Baltimore, MD, USA

Abstract

We assessed the interactive effect of genetic polymorphisms and exercise training on fibrinolysis in 50 - 75 yr old men (n = 17) and women (n = 28). Subjects had tissue plasminogen activator (t-PA) antigen levels and activity and plasminogen activator inhibitor-1 (PAI-1) activity measured before and after 6 mo of endurance-exercise training. Subject's DNA was typed for the PAI-1 4 G/5 G and t-PA I/D variants. Baseline PAI-1 activity, t-PA activity, and t-PA antigen levels were not different among PAI-1 or t-PA genotype groups. Overall, exercise training did not change PAI-1 activity (- 0.43 ± 0.81 IU/mL, p = NS), increased t-PA activity (0.37 ± 0.16 IU/mL, p = 0.02), and decreased t-PA antigen levels (- 0.88 ± 0.20 ng/mL, p < 0.001). Although the differences in changes with training were not significant among genotype groups, significant t-PA antigen level improvements were evident only in PAI-1 4 G allele carriers and significant t-PA activity increases only in PAI-1 4 G homozygotes. t-PA genotype affected the training-induced t-PA antigen level improvements (p = 0.033) after covarying for gender and baseline t-PA antigen levels, with the smallest and largest reductions in the D homozygotes and I/D heterozygotes, respectively. These findings could have important treatment implications for the use of exercise training to reduce CV disease and thrombotic risk in older men and women.

Key words

PAI-1 activity - t-PA antigen - PAI-1 genotype - t-PA genotype

Full Text

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PhD James Hagberg

Department of Kinesiology
University of Maryland

College Park, MD 20742-2611

USA

Phone: + 3014052487

Fax: + 30 14 05 55 78

Email: hagberg@umd.edu