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Horm Metab Res 2005; 37(7): 402-407
DOI: 10.1055/s-2005-870228
Original Basic
© Georg Thieme Verlag KG Stuttgart · New York

Effect of High-fat Diet on KKAy and ob/ob Mouse Liver and Adipose Tissue Corticosterone and 11-dehydrocorticosterone Concentrations

P.  Alberts1 , Y.  Rönquist-Nii1 , C.  Larsson1 , G.  Klingström1 , L.  Engblom1 , N.  Edling1 , V.  Lidell1 , I.  Berg1 , P.-O.  Edlund1 , M.  Ashkzari1 , N.  Sahaf1 , S.  Norling1 , V.  Berggren1 , K.  Bergdahl1 , M.  Forsgren1 , L.  Abrahmsén1
  • 1Department of Biology, Biovitrum, Stockholm, Sweden
Further Information

Publication History

Received 30 September 2004

Accepted after revision 15 February 2005

Publication Date:
21 July 2005 (online)

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Abstract

The present study was performed to compare glucocorticoid levels in obese KKAy and ob/ob mice with those in normal C57BL/6J mice, and the effect of high-fat diet on glucocorticoids in KKAy and ob/ob mice. Liver, mesenteric and epididymal adipose tissue corticosterone and 11-dehydrocorticosterone concentrations as well as circulating corticosterone concentrations were measured. The KKAy and ob/ob mice displayed elevated serum corticosterone levels compared to normal mice, 2.0 to 2.8-fold in KKAy, and 11 to 16-fold in ob/ob mice. Liver corticosterone levels were 3.0 to 5.1 and 6.2 to 8.1-fold, and 11-dehydrocorticosterone levels were 3.4 to 3.6 and 6.7 to 8.2-fold higher in KKAy and ob/ob mice compared to normal mice. Mesenteric adipose tissue corticosterone levels were 2.7 to 4.2-fold higher, and 11-dehydrocorticosterone levels were 2 to 4-fold higher in ob/ob than in KKAy mice. Epididymal adipose tissue corticosterone levels were 3.0 to 6.2-fold higher, and 11-dehydrocorticosterone levels were 1.8 to 2.0-fold higher in ob/ob than in KKAy mice. Circulating, hepatic, and mesenteric and epididymal adipose tissue glucocorticoid concentrations were low in the normal C57BL/6J mouse, high in the ob/ob mouse, and intermediate in the KKAy mouse. 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) mRNA levels were doubled in ob/ob compared to KKAy mice in all three tissues. Glucocorticoid concentrations correlated with 11β-HSD1 mRNA levels. High-fat diet had no effect on the tissue glucocorticoid concentrations.

Key words

11-Dehydrocorticosterone - Corticosterone - Diabetes - Glucocorticoids - 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) - Metabolic syndrome - Obesity

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P. Alberts *

Pharmacology 2, Department of Biology

Biovitrum SE12 · SE 112 76 Stockholm · Sweden

* Present address: Affibody, Box 20137, SE-161 02 Bromma, Sweden

Phone: +46 (70) 322 3046 ·

Fax: +46 (8) 697 34 36

Email: peteris.alberts@biovitrum.com