Effect of Three Flavonoids Isolated from Japanese Polygonum Species on Superoxide Generation in Human Neutrophils

Abstract

Three flavonoids, quercetin 3-O-glucoside-2′′-gallate (QGG), quercetin 3-O-rhamnoside-2′′-gallate (QRG) and kaempferol 3-O-glucoside-2′′-gallate (KGG) were isolated from Japanese Polygonum species. The effect of these flavonoids on stimulus-induced superoxide generation in human neutrophils was assayed by measuring the reduction of cytochrome c. The tyrosyl or serine/threonine phosphorylation of neutrophil proteins and the translocation of p47^phox and p67^phox to the cell membrane were detected using specific monoclonal antibodies. The flavonoids used in this experiment significantly suppressed stimulus-induced superoxide generation in a concentration-dependent manner. FMLP-induced tyrosyl phosphorylation or PMA-induced serine/threonine phosphorylation and the translocation of the cytosolic proteins p47^phox and p67^phox to the cell membrane were suppressed in parallel to the suppression of the stimulus-induced superoxide generation.

Polygonum - flavonoids - superoxide - human neutrophils - cytosolic compounds

References

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