Abstract
Overproduction of nitric oxide (NO) by inducible nitric oxide synthase (iNOS) acts as a neurotoxic effector in the central nervous system, resulting in neurodegenerative diseases. From the alcoholic extracts of Perilla frutescens, we have purified an inhibitor of NO production in lipopolysaccharide (LPS)-activated microglia by activity-guided purification. The active compound was identified as luteolin by spectral analysis. Luteolin inhibited the NO production in LPS-activated microglia in a dose-dependent manner (IC50 = 6.9 μM). Luteolin also suppressed the degradation of I-κB-α, the expression of protein and mRNA of iNOS in LPS-activated microglia as observed in Western blot analysis and RT-PCR experiments. Luteolin may have beneficial effects in the treatment of neuro-inflammatory diseases through the inhibition of iNOS expression.
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Jae-Ha Ryu
College of Pharmacy
Sookmyung Women’s University
53-12 Chungpa-Dong
Yongsan-Gu
Seoul 140-742
Korea
Phone: +82-2-710-9568
Fax: +82-2-714-0745
Email: ryuha@sookmyung. ac.kr
