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Planta Med 2006; 72(2): 114-120
DOI: 10.1055/s-2005-873148
Original Paper
Pharmacology
© Georg Thieme Verlag KG Stuttgart · New York

Protection of the Hemopoietic System by Podophyllum hexandrum against Gamma Radiation-Induced Damage

Ravinder Kumar Sagar1 , Raman Chawla1 , Rajesh Arora1 , Shikha Singh1 , Bal Krishna3 , Rakesh Kumar Sharma1 , Satish Chandra Puri2 , Pankaj Singh1 , Raj Kumar1 , Ashok Kumar Sharma1 , Surender Singh1 , Jagdish Prasad1 , Vinay Gupta2 , Bilal Ahmed2 , Kanaya Lal Dhar2 , Haider Ali Khan4 , Manju Lata Gupta1 , Ghulam Nabi Qazi2
  • 1Department of Radiation Biology, Institute of Nuclear Medicine and Allied Sciences, Delhi, India
  • 2Natural Products Chemistry Division, Regional Research Laboratory (CSIR), Jammu, India
  • 3Department of Physiology, Maulana Azad Medical College, University of Delhi, Delhi, India
  • 4Jamia Hamdard, Hamdard Nagar, New Delhi, India
Further Information

Publication History

Received: April 26, 2005

Accepted: June 20, 2005

Publication Date:
10 November 2005 (online)

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Protection of the Hematopoetic System by Podophyllum hexandrum against Gamma Radiation-Induced DamagePlanta Med 2006; 72(03): 288-288
DOI: 10.1055/s-2006-931539

Abstract

A semi-purified extract of low-altitude Podophyllum hexandrum (REC-2001) containing a relatively low content of podophyllotoxin (3.25 %) exhibited potent antioxidant ability in lipid media (at 1000 μg/mL against 0.25 kGy) and significant (p < 0.05) hydroxyl ion scavenging potential (78.83 % at 500 μg/mL). In vitro investigations revealed the ability of REC-2001 to significantly (p < 0.05) reduce radiation-induced hemolysis (2 μg/mL; 46.184 %) and nitric oxide scavenging levels (IC50: 792 ± 1.25 μg/mL). Protection of the hemopoietic system of Strain ‘A’ mice administered 20 mg/kg BW REC-2001 30 min prior to lethal irradiation (10 Gy) was recorded and was mediated by free radical scavenging and lowering of lipid oxidation. Further studies investigating the effects of REC-2001 on stem cell modulation are warranted.

Key words

Radioprotection - hemopoietic system - antioxidant - Podophyllum hexandrum - Podophyllotoxin - lignan glycoside

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Dr. Rakesh Kumar Sharma

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