Abstract
A micellar electrokinetic capillary chromatography (MEKC) method for the separation and quantification of the coumarins in Cnidii Fructus was developed for the first time. Within 25 minutes, 5 major coumarins were separated using 18 mM borate, 12 mM phosphate and 50 mM SDS (pH 9.2) containing 20 % methanol. The relative standard deviations of migration times and peak areas were less than 5 %. The total contents of the five coumarins in plants from different habitats ranged from 13.6 to 44.6 mg/g, and recoveries ranged from 96.0 to 102.8 %. The effects of buffer concentration, SDS, pH value and organic modifier on the separation were investigated.
Key words
Capillary electrophoresis - micellar electrokinetic capillary chromatography - coumarins -
Cnidium monnieri
- Umbelliferae
References
-
1 Jiangsu New Medical C ollege. Zhong Yao Da Ci Dian (Dictionary of Chinese Materia Medica). Shanghai; Shanghai Scientific and Technological Publisher 1977: pp 2121-3
-
2
Kimiye B, Fumiyo H, Yuko T, Mitsugi K, Gisho H, Mamoru T.
Chemical studies on Chinese crude drug ”She chuang zi”.
Shoyakugaku Zasshi.
1985;
39
282-90
-
3
Kiyoshi H, Mitsugi K, Kimiye B.
Coumarins from the Chinese crude drug ”She chuang zi”, the fruits of Cnidium sp. and from Cnidium japonicum Miq.
Shokubutsu Kenkyu Zasshi.
1972;
47
326-8
-
4
Gisho H, Mamoru T, Kimiye B, Mitsugi K.
On the antidermatophytic constituents and the original plants of the traditional Chinese drug ”She chuang zi”.
Shoyakugaku Zasshi.
1984;
38
221-6
-
5 Mcevoy G K. American Hospital Formulary Service - Drug Information 93. Bethesda; American Society of Hospital Pharmacists, Inc 1993: p 2267
-
6
Cai Y, Bennett D, Nair R V, Ceska O, Ashwood-Smith M J, DiGiovanni J.
Inhibition and inactivation of murine hepatic ethoxy- and pentoxyresorufin O-dealkylase by naturally occurring coumarins.
Chem Res Toxicol.
1993;
6
872-9
-
7
Kleiner H E, Vulimiri S V, Miller L, Johnson W H, Whitman C P, DiGiovanni J.
Oral administration of naturally occurring coumarins leads to altered phase I and II enzyme activities and reduced DNA adduct formation by polycyclic aromatic hydrocarbons in various tissues of SENCAR mice.
Carcinogenesis.
2001;
22
73-82
-
8
Mengeaud V, Ortonne J P.
Regulation of melanogenesis induced by 5-methoxypsoralen without ultraviolet light in murine melanoma cells.
Pigment Cell Res.
1994;
7
245-54
-
9
Li X X, Hara I, Matsumiya T.
Effects of osthole on postmenopausal osteoporosis using ovariectomized rats; comparison to the effects of estradiol.
Biol Pharm Bull.
2002;
25
738-42
-
10
Tomas-Barberan F A.
Capillary electrophoresis: a new technique in the analysis of plant secondary metabolites.
Phytochem Anal.
1995;
6
177-92
-
11
Ganzera M, Sturm S, Stuppner H.
HPLC-MS and MECC analysis of coumarins.
Chromatographia.
1997;
46
197-203
-
12
Ochocka R J, Rajzer D R, Kowalski P, Lamparczyk H.
Determination of courmarins from Chrysanthemum segetum L. by capillary electrophoresis.
J Chromatogr A.
1995;
709
197-202
-
13
Aucamp J P.
Determination of scoparone in Citrus roots by micellar electrokinetic capillary chromatography.
HRC - J High Res Chromatogr.
2000;
23
519-21
-
14
Kazuhiko S, Toshiyuki O, Satoshi S, Tsuguchika Y.
Determination of coumarins in Cnidium monnieri fructus by high-performance liquid chromatography.
J Chromatogr.
1987;
388
448-54
-
15
Cherng T C, Shuenn J S.
Separation of coumarins by micellar electrokinetic capillary chromatography.
J Chromatogr A.
1995;
710
323-9
-
16
Shu F W, Yong J, Xing G C, Zhi D H.
Separation and determination of coumarins in the root bark of three Citrus plants by micellar electrokinetic capillary chromatography.
Planta Med.
2003;
69
483-6
-
17
Otsuka K, Higashimori M, Koike R, Karuhaka K, Okada Y, Terabe S.
Separation of lipophilic compounds by micellar electrokinetic chromatography with organic modifiers.
Electrophoresis.
1994;
15
1280-3
-
18
Yang S S, Smetena I, Goldsmith A I.
Evaluation of micellar electrokinetic capillary chromatography for the analysis of selected tobacco alkaloids.
J Chromatogr A.
1996;
746
131-6
-
19
Wu M H, Huang C Y, Zhao L H, Mei L H.
Studies on the HPLC fingerprints of fructus Cnidii
.
Chin J Nat Med.
2005;
3
106-11
-
20
Basnet P, Yasuda I, Kumagai N, Tohda C, Nojima H, Kuraishi Y. et al .
Inhibition of itch-scratch response by fruits of Cnidium monnieri in mice.
Biol Pharm Bull.
2001;
24
1012-5
Lu Hua Zhao
Key Laboratory of Modern Chinese Medicine
Ministry of Education
Nanjing
People’s Republic of China
Telefon: +86-25-8327-1185
Fax: +86-25-8327-1399
eMail: zhaoluhua@hotmail.com
