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Planta Med 2006; 72(1): 46-51
DOI: 10.1055/s-2005-873181
Original Paper
Natural Product Chemistry
© Georg Thieme Verlag KG Stuttgart · New York

Xanthine Oxidase Inhibitors from the Flowers of Chrysanthemum sinense

Mai Thanh Thi Nguyen1 , Suresh Awale1 , Yasuhiro Tezuka1 , Jun-ya Ueda1 , Quan Le Tran2 , Shigetoshi Kadota1
  • 1Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Toyama, Japan
  • 2National University-Hochiminh City, Hochiminh City, Vietnam
Further Information

Publication History

Received: March 21, 2005

Accepted: June 10, 2005

Publication Date:
10 November 2005 (online)

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Abstract

From the MeOH extract of the flowers of Chrysanthemum sinense, a new flavone glucoside, acacetin 7-O-(3-O-acetyl-β-D-glucopyranoside) (1), has been isolated together with 27 known compounds including flavonoids, caffeoylquinic acid derivatives, phenolics, and a monoterpenoid glucoside. Their structures were elucidated on the basis of spectroscopic data. Compounds 1 - 15, 20 - 24, and 27 displayed significant xanthine oxidase inhibitory activity in a concentration-dependent manner, and compounds 2 - 11 and 22 showed more potent inhibitory activity, with IC50 values ranging from 0.13 to 2.31 μM, than that of a positive control allopurinol (IC50 = 2.50 μM). The kinetic study indicated that 1 - 15 and 20 - 24 displayed competitive-type inhibition like that of allopurinol, while 27 displayed a mixed-type inhibition.

Key words

Chrysanthemum sinense - Asteraceae - acacetin 7-O-(3-O-acetyl-β-D-glucopyranoside) - flavonoids - caffeoylquinic acid - phenolics - Xanthine oxidase inhibition

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Prof. Dr. Shigetoshi Kadota

Institute of Natural Medicine

Toyama Medical and Pharmaceutical University

2630 Sugitani

Toyama 930-0194

Japan

Phone: +81-76-434-7625

Fax: +81-76-434-5059

Email: kadota@ms.toyama-mpu.ac.jp