Xanthine Oxidase Inhibitors from the Flowers of Chrysanthemum sinense

Abstract

From the MeOH extract of the flowers of Chrysanthemum sinense, a new flavone glucoside, acacetin 7-O-(3-O-acetyl-β-D-glucopyranoside) (1), has been isolated together with 27 known compounds including flavonoids, caffeoylquinic acid derivatives, phenolics, and a monoterpenoid glucoside. Their structures were elucidated on the basis of spectroscopic data. Compounds 1 - 15, 20 - 24, and 27 displayed significant xanthine oxidase inhibitory activity in a concentration-dependent manner, and compounds 2 - 11 and 22 showed more potent inhibitory activity, with IC₅₀ values ranging from 0.13 to 2.31 μM, than that of a positive control allopurinol (IC₅₀ = 2.50 μM). The kinetic study indicated that 1 - 15 and 20 - 24 displayed competitive-type inhibition like that of allopurinol, while 27 displayed a mixed-type inhibition.

Chrysanthemum sinense - Asteraceae - acacetin 7-O-(3-O-acetyl-β-D-glucopyranoside) - flavonoids - caffeoylquinic acid - phenolics - Xanthine oxidase inhibition

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