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DOI: 10.1055/s-2005-873199
© Georg Thieme Verlag KG Stuttgart · New York
New Lignans and Cytotoxic Constituents from Schisandra propinqua
Publikationsverlauf
Received: April 5, 2005
Accepted: July 13, 2005
Publikationsdatum:
08. Dezember 2005 (online)
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Abstract
The CHCl3 extract from the stems of Schisandra propinqua (Wall.) Baill exhibited significant cytotoxic activity against cancer cell lines. Subsequent fractionation procedures led to the isolation of four new dibenzocyclooctadiene lignans, propinquanins A - D, along with two known lignans, schisantherin I and heteroclitin A, and three known triterpenoids, isoschisandrolic acid, schisandrolic acid and schisandronic acid. Their structures, including absolute stereochemistry, were elucidated through analyses of NMR data and CD spectra. The isolated compounds were tested for their cytotoxic activity in several tumor cell lines with the MTT assay. Propinquanin B (2) was significantly cytotoxic (IC50 values < 10μM) in HL-60 and Hep-G2 tumor cell lines. Cell cycle analysis and Hoechst 33 258 staining assay suggested that one possible mechanism of cytotoxic activity by 2 might be related to the induction of apoptosis.
Key words
Schisandra propinqua (Wall.) Baill - Schisandraceae - dibenzocyclooctadiene - lignans - triterpenoids - cytotoxictity
- Supporting Information for this article is available online at
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Prof. Shi-Lin Chen
Department of Applied Biology & Chemical Technology
The Hong Kong Polytechnic University
Hung Hom
Hong Kong
People’s Republic of China
eMail: bcslchen@inet.polyu.edu.hk
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