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Horm Metab Res 2005; 37(12): 722-728
DOI: 10.1055/s-2005-921092
Original Basic
© Georg Thieme Verlag KG Stuttgart · New York

Differential Expression of the Human Somatostatin Receptor Subtypes sst1 to sst5 in Various Adrenal Tumors and Normal Adrenal Gland

B.  Ueberberg1 , H.  Tourne1 , A.  Redman1 , M.  K.  Walz2 , K.  W.  Schmid3 , K.  Mann1 , S.  Petersenn1
  • 1Division of Endocrinology, Medical Center, University of Essen, Essen, Germany
  • 2Department of Surgery and Center of Minimally Invasive Surgery, Kliniken Essen-Mitte, Essen, Germany
  • 3Institute of Pathology, University of Essen, Essen, Germany
Weitere Informationen

Publikationsverlauf

Received 7 March 2005

Accepted after revision 9 August 2005

Publikationsdatum:
22. Dezember 2005 (online)

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Erratum zu diesem Artikel:

ErratumHorm Metab Res 2006; 38(09): 617-617
DOI: 10.1055/s-2006-956173

Abstract

Somatostatin (SRIF) is a widely distributed peptide with growth-inhibiting effects in various tumors. So far, five distinct human SRIF receptor subtypes (sst1 - sst5) have been identified. We investigated expression of the five ssts in various adrenal tumors and in normal adrenal gland. Tissue was obtained from ten pheochromocytomas (PHEOs), nine cortisol-secreting adenomas (CPAs), eleven aldosterone secreting adenomas (APAs) and eight non-functional adenomas (NFAs) after retroperitoneoscopic surgery, and used for RNA extraction. Adrenal tissue surrounding the tumor was available for analysis in twenty-seven cases. Receptor expression was studied by RT-PCR using sst-specific primers and subsequently confirmed by Southern blotting. Expression of all five receptor subtypes was observed in RNA obtained from normal adrenal gland. Furthermore, each receptor subtype was expressed in more than 50 % of all tumors analyzed. No sst5 expression was found in PHEOs, while sst1 was present in nearly all of these tumors. Only a few of the CPAs expressed subtypes sst1 and sst4. Expression of all five subtypes was distributed equally in APAs. No sst4 was found in any of the NFAs. Differential expression of ssts in various adrenal tumors may point to new aspects in the pathogenesis of these adenomas. Furthermore, the presence of specific ssts could expand the diagnostic and therapeutic strategies during management. New subtype specific analogues of SRIF may be used in the future depending on the type of adrenal tumor and receptor subtype expressed.

Key words

Somatostatin receptor - somatostatin analogues - adrenal gland - adrenal tumor - pheochromocytoma - aldosterone - cortisol - expression

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PD Dr. med. Stephan Petersenn

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