PDF herunterladen
Horm Metab Res 2006; 38(5): 336-340
DOI: 10.1055/s-2006-925407
Original Clinical
© Georg Thieme Verlag KG Stuttgart · New York

Association of Serum Adiponectin Concentration to Lipid and Glucose Metabolism in Healthy Humans

M.  K.  Heliövaara1 , T.  E.  Strandberg1 , S.-L.  Karonen2 , P.  Ebeling1
  • 1 Department of Medicine, Division of Geriatrics, Helsinki University Central Hospital, Helsinki, Finland
  • 2 Department of Clinical Physiology, Helsinki University Central Hospital, Helsinki, Finland
Weitere Informationen

Publikationsverlauf

Received 15 August 2005

Accepted after revision 19 December 2005

Publikationsdatum:
23. Mai 2006 (online)

Auch verfügbar auf
    Lizenzen und Reprints

Abstract

Background: Adiponectin is a recently discovered plasma protein with many associations to glucose and lipid metabolism. Due to its central role in cardiovascular diseases and insulin resistance, we studied the relationship between serum adiponectin and factors reflecting glucose and lipid metabolism. Methods and Results: Thirty healthy participants (20M/10F, age 32.0 ± 2.1 years, BMI 25.8 ± 0.9 kg/m2 and HbA1c 5.2 ± 0.1 %) were studied four times at approximately one week intervals. The effects of a 4-hour euglycemic hyperinsulinemia (40 mU/m2/min), saline infusion (control), oral glucose, and oral fat load on serum adiponectin were studied. No significant correlation was found between serum adiponectin and insulin sensitivity before (r = 0.25) or after adjustment for age, BMI and gender (r = 0.04). Adiponectin concentration correlated inversely with HbA1c (r = - 0.43, p < 0.05), insulin concentration (r = - 0.38, p < 0.05) and triglyceride concentration (r = - 0.42, p < 0.05) but positively with HDL cholesterol (r = 0.38, p < 0.05). Metabolic procedures had no effect on serum adiponectin. Conclusions: Our findings favor the interpretation that adiponectin is not causally related to insulin sensitivity in healthy participants. The strongest associations of adiponectin in healthy participants are to be found to lipid metabolism. Serum levels of adiponectin are very stable and not acutely affected by hyperinsulinemia, oral glucose or fat load.

Key words

Adiponectin - insulin sensitivity - HbA1c - triglycerides - HDL cholesterol

References

  • 1 Hu E, Liang P, Spiegelman B M. AdipoQ is a novel adipose-specific gene dysregulated in obesity.  J Biol Chem. 1996;  271 10 697-10 703
    PubMedSuche in Google Scholar
  • 2 Shapiro L, Scherer P E. The crystal structure of a complement-1q family protein suggests an evolutionary link to tumor necrosis factor.  Curr Biol. 1998;  8 335-338
    CrossrefPubMedSuche in Google Scholar
  • 3 Ouchi N, Kihara S, Arita Y, Maeda K, Kuriyama H, Okamoto Y, Hotta K, Nishida M, Takahashi M, Nakamura T, Yamashita Y, Funahashi T, Matsuzawa Y. Novel modulator for endothelial adhesion molecules. Adipocyte-derived plasma protein adiponectin.  Circulation. 1999;  100 2473-2476
    CrossrefPubMedSuche in Google Scholar
  • 4 Ouchi N, Kihara S, Arita Y, Okamoto Y, Maeda K, Kuriyama H, Hotta K, Nishida M, Takahashi M, Muraguchi M, Ohmoto Y, Nakamura T, Yamashita S, Funahashi T, Matsuzawa . Adiponectin, an adipocyte-derived plasma protein inhibits endothelial NF-kB signaling through a cAMP-dependent pathway.  Circulation. 2000;  102 1296-1301
    CrossrefPubMedSuche in Google Scholar
  • 5 Yokota T, Oritani K, Takahashi I, Ishikawa J, Matsuyama A, Ouchi N, Kihara S, Funahashi T, Tenner J A, Tomiyama Y, Matsuzawa Y. Adiponectin, a new member of the family of soluble defense collagens negatively regulates the growth of myelomonocytic progenitors and the functions of macrophages.  Blood. 2000;  96 1723-1732
    PubMedSuche in Google Scholar
  • 6 Arita Y, Kihara S, Ouchi N, Takahashi M, Maeda K, Miyagawa J-I, Hotta K, Shimomura I, Nakamura T, Miyaoka K, Kuriyama H, Nishida M, Yamashita Y, Okubo K, Matsubara K, Muraguchi M, Ohmoto Y, Funahashi T, Matsuzawa Y. Paradoxical decrease of an adipose-specific protein adiponectin in obesity.  Biochem Biophys Res Commun. 1999;  257 79-83
    CrossrefPubMedSuche in Google Scholar
  • 7 Hotta K, Funahashi T, Arita Y, Takahashi M, Matsuda M, Okamoto Y, Iwahashi H, Kuriyama H, Ouchi N, Maeda K, Nishida M, Kihara S, Sakai N, Nakajima T, Hasegawa K, Muraguchi M, Ohmoto Y, Nakamura T, Yamashita S, Hanafusa T, Matsuzawa Y. Plasma concentrations of a novel adipose-specific protein, adiponectin in type 2 diabetic patients.  Arterioscl Thromb Vasc Biol. 2000;  20 1595-1599
    CrossrefPubMedSuche in Google Scholar
  • 8 Weyer C, Funahashi T, Tanaka S, Hotta K, Matsuzawa Y, Pratley R E, Tataranni P A. Hypoadiponectemia in obesity and type 2 diabetes: close association with insulin resistance and hyperinsulinemia.  J Clin Endocrinol Metab. 2001;  86 1930-1935
    CrossrefPubMedSuche in Google Scholar
  • 9 Yamauchi T, Kamon J, Waki H, Terauchi Y, Kubota N, Hara K, Mori Y, Ide T, Murakami K, Tsuboyama-Kasaoka N, Ezaki O, Akanuma Y, Gavrilova O, Vinson C, Reitman M L, Kagechika H, Shudo K, Yoda M, Nakano Y, Tobe K, Nagai R, Kimura S, Tomita M, Froguel P, Kadowaki T. The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity.  Nature Med. 2001;  7 941-946
    CrossrefPubMedSuche in Google Scholar
  • 10 Berg A H, Combs T P, Du X, Brownlee M, Scherer P E. The adipocyte-secreted protein Acrp30 enhances hepatic insulin action.  Nature Med. 2001;  7 947-953
    CrossrefPubMedSuche in Google Scholar
  • 11 Ebeling P, Bourey R, Koranyi L, Tuominen J A, Groop L C, Henriksson J, Mueckler M, Sovijärvi A, Koivisto V A. Mechanisms of enchanced insulin sensitivity in athletes.Increased blood flow, muscle glucose transport protein(GLUT-4) concentration, and glycogen synthase activity.  J Clin Invest. 1993;  92 1623-1631
    PubMedSuche in Google Scholar
  • 12 DeFronzo R A, Tobin J D, Andres R. Glucose clamp technique: a method for quantifying insulin secretion and resistance.  Am J Physiol. 1979;  237 E214-E223
    PubMedSuche in Google Scholar
  • 13 McGuire E AH, Helderman J H, Tobin J D, Andres R, Berman M. Effects of arterial versus venous sampling on analysis of glucose kinetics in man.  J Appl Physiol. 1976;  41 565-573
    PubMedSuche in Google Scholar
  • 14 Koivisto V A, Yki-Järvinen H, Puhakainen I, Virkamäki A, Kolaczynski J, DeFronzo R. No evidence for isotope discrimination of tritiated glucose tracers in measurements of glucose turnover rates in man.  Diabetologia. 1990;  33 168-173
    CrossrefPubMedSuche in Google Scholar
  • 15 Ferrannini E. The theoretical bases of indirect calorimetry: a review.  Metabolism. 1988;  37 287-301
    CrossrefPubMedSuche in Google Scholar
  • 16 Hotta K, Funahashi T, Bodkin N L, Ortmeyer H K, Arita Y, Hansen B C, Matsuzawa Y. Circulating concentrations of the adipocyte protein adiponectin are decreased in parallel with reduced insulin sensitivity during the progression to type 2 diabetes in rhesus monkeys.  Diabetes. 2001;  50 1126-1133
    PubMedSuche in Google Scholar
  • 17 Weyer C, Tataranni P A, Pratley R E. Insulin action and insulinemia are closely related to the fasting complement C3, but not to acylation stimulating protein concentration.  Diabetes Care. 2000;  23 779-785
    PubMedSuche in Google Scholar
  • 18 Stefan N, Vozarova B, Funahashi T, Matsuzawa Y, Weyer C, Lindsay R S, Yougren J F, Havel P J, Pratley R E, Bogardus C, Tataranni P A. Plasma adiponectin concentration is associated with skeletal muscle insulin receptor tyrosin phosphorylation and low plasma concentration precedes a decrease in whole-body insulin sensitivity in humans.  Diabetes. 2002;  50 1884-1888
    PubMedSuche in Google Scholar
  • 19 Baratta R, Amato S, Degano C, Degano C, Farina M G, Patane G, Vigneri R, Frittitta L. Adiponectin relationship with lipid metabolism is independent of body fat mass: Evidence from both cross-sectional and intervention studies.  J Clin Endocrinol Metab. 2004;  89 2665-2671
    CrossrefPubMedSuche in Google Scholar
  • 20 Ma K, Cabrero A, Saha P K, Kojima H, Li L, Chang B H, Paul A, Chan L. Increased beta-oxidation but no insulin resistance or glucose intolerance in mice lacking adiponectin.  J Biol Chem. 2002;  277 34 658-34 661
    PubMedSuche in Google Scholar
  • 21 Maeda N, Shimomura I, Kishida K, Nishizawa H, Matsuda M, Nagaretani H, Furuyama M, Kondo H, Takahashi M, Arita Y, Komuro R, Ouchi N, Kihara S, Tochino Y, Okutomi K, Horie M, Takeda S, Aoyama T, Funahashi T, Matsuzawa Y. Diet-induced insulin resistance in mice lacking adiponectin/ACRP30.  Nature Med. 2002;  8 731-737
    CrossrefPubMedSuche in Google Scholar
  • 22 Snieder H, Sawtell P A, Ross L, Walker J, Spector T D, Leslie R DG. HbA1c levels are genetically determined even in Type 1 diabetes. Evidence from healthy and diabetic twins.  Diabetes. 2001;  50 2858-2863
    CrossrefPubMedSuche in Google Scholar
  • 23 Matsubara M, Maruoka S, Katayose S. Decreased plasma adiponectin concentrations in women with dyslipidemia.  J Clin Endocrinol Metab. 2002;  87 2764-2769
    CrossrefPubMedSuche in Google Scholar
  • 24 Beutler B A, Cerami A. Recombinant interleukin 1 suppresses lipoprotein lipase activity in 3T3-L1 cells.  J Immunol. 1985;  135 3969-3971
    PubMedSuche in Google Scholar
  • 25 Sammalkorpi K, Valtonen V, Kerttula Y, Nikkilä E, Taskinen M-R. Changes in serum lipoprotein pattern induced by acute infections.  Metabolism. 1988;  37 859-865
    CrossrefPubMedSuche in Google Scholar
  • 26 Sammalkorpi K, Valtonen V, Maury C PJ. Lipoproteins and acute phase response during acute infection. Interrelationships between C-reactive protein and serum amyloid-A protein and lipoproteins.  Ann Med. 1990;  22 397-401
    PubMedSuche in Google Scholar
  • 27 Yang W-S, Lee W-J, Funahashi T, Tanaka S, Matsuzawa Y, Chao C-L, Chen C-L, Tai T-Y, Chuang L-M. Weight reduction increases plasma levels of an adipose-derived anti-inflammatory protein adiponectin.  J Clin Endocrinol Metab. 2001;  86 3815-3819
    CrossrefPubMedSuche in Google Scholar
  • 28 Hirose H, Kawai T, Yamamoto Y, Taniyama M, Tomita M, Matsubara K, Okazaki Y, Ishii T, Oguma Y, Takei I, Saruta T. Effects of pioglitazone on metabolic parameters, body fat distribution and serum adiponectin levels in Japanese male patients with type 2 diabetes.  Metabolism. 2002;  51 314-317
    CrossrefPubMedSuche in Google Scholar
  • 29 Halleux C M, Takahashi M, Delporte M L, Destry R, Funahashi T, Matsuzawa Y, Brichard S M. Secretion of adiponectin and regulation of apM1 gene expression in human visceral adipose tissue.  Biochem Biophys Res Commun. 2001;  288 1102-1107
    CrossrefPubMedSuche in Google Scholar
  • 30 Fasshauer M, Klein J, Neumann S, Eszlinger M, Paschke R. Hormonal regulation of adiponectin gene expression in 3T3-L1 adipocytes.  Biochem Biophys Res Commun. 2002;  290 1084-1089
    CrossrefPubMedSuche in Google Scholar
  • 31 Bogan J S, Lodish H F. Two compartments for insulin-stimulated exocytosis in 3T3-L1 adipocytes defined by endogenous ACRP30 and GLUT4.  J Cell Biol. 1999;  146 609-620
    CrossrefPubMedSuche in Google Scholar

Maikki K. Heliövaara, M.D.

Helsinki University Central Hospital · Department of Medicine ·

P.O. Box 340 · FIN-00029 HYKS · Finland

Telefon: +358 (9) 47 17 45 07

Fax: +358 (9) 47 17 55 44 ·

eMail: maikki.heliovaara@hus.fi