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DOI: 10.1055/s-2006-931561
© Georg Thieme Verlag KG Stuttgart · New York
Valerian Extract Ze 911 Inhibits Postsynaptic Potentials by Activation of Adenosine A1 Receptors in Rat Cortical Neurons
Publication History
Received: February 3, 2005
Accepted: January 16, 2006
Publication Date:
28 April 2006 (online)
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Abstract
In this study we evaluated the adenosine A1 receptor-mediated effect of valerian extract (Ze 911) on postsynaptic potentials (PSPs) in pyramidal cells of the rat cingulate cortex in a slice preparation. We first observed that N 6-cyclopentyladenosine (CPA, 0.01 - 10 μM), an adenosine A1 receptor agonist, inhibited PSPs in a concentration-dependent manner. The CPA (10 μM)-induced inhibition was antagonized by 1,3-dipropyl-8-cyclopentylxanthine (DPCPX, 0.1 μM), an adenosine A1 receptor antagonist. Ze 911 concentration dependently (0.1 - 15 mg/mL) inhibited PSPs in the presence of the adenosine A2A receptor antagonist 1,3,7-trimethyl-8-(3-chlorostyryl)xanthine (CSC, 0.2 μM) and adenosine deaminase (1 U/mL). The maximal inhibition induced by 10 mg/mL was completely antagonised by DPCPX (0.1 μM), an A1 receptor blocker. The data suggest that activation of adenosine A1 receptors is involved in the pharmacological effects of the valerian extract Ze 911.
Key words
Adenosine - adenosine A1 receptor - valerian - postsynaptic potential - pyramidal cells
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Dr. Karen Nieber
Universität Leipzig
Institut für Pharmazie
Talstrasse 33
04103 Leipzig
Germany
Phone: +49-341-973-6812
Fax: +49-341-973-6898
Email: nieber@rz.uni-leipzig.de