Planta Med 2006; 72(7): 615-620
DOI: 10.1055/s-2006-931570
Original Paper
Pharmacology
© Georg Thieme Verlag KG Stuttgart · New York

N-Acetylphenylisoserinates of Lactarius Sesquiterpenoid Alcohols - Cytotoxic, Antiviral, Antiproliferative and Immunotropic Activities in vitro

Ewa Krawczyk1 , 3 , Monika Kniotek2 , Maria Nowaczyk2 , Tomasz Dzieciatkowski1 , Maciej Przybylski1 , Anna Majewska1 , Miroslaw Luczak1
  • 1Department of Medical Microbiology, Medical University of Warsaw, Warsaw, Poland
  • 2Department of Clinical Immunology, Transplantation Institute, Medical University of Warsaw, Warsaw, Poland
  • 3Current address: Department of Pathology, Georgetown University Medical Center , Preclinical Sciences Building, Washington, DC, USA
Further Information

Publication History

Received: November 27, 2005

Accepted: January 27, 2006

Publication Date:
24 April 2006 (online)

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Abstract

In the present study a new group of derivatives of both paclitaxel and Lactarius sesquiterpenes, the N-acetylphenylisoserinates of Lactarius sesquiterpenoid alcohols, was tested for antiviral, cytotoxic, antiproliferative and immunotropic activities in vitro. Among the 13 compounds tested two, isolactarorufin 8-[N-acetyl-(2′R,3′S)-3′-phenylisoserinate] and 3-O-ethylfurandiol 8-[N-acetyl-(2′R,3′S)-3′-phenylisoserinate] inhibited Herpes simplex virus type 1 (HSV-1) replication at low cytotoxic concentrations. Selectivity indices were: > 12.2 and > 106.4, respectively. Both compounds decreased also the number of cell divisions. Mitotic indices of the cells submitted to these compounds were: 96/2000 and 48/1000, respectively, in comparison with control (169/2000). It seems that the antimitotic action of the compounds may be associated with their antiviral activity. Moreover, isolactarorufin 8-[N-acetyl-(2′R,3′S)-3′-phenylisoserinate] inhibited PHA-induced T lymphocyte proliferation.

Abbreviations

MEM:minimum essential medium

FCS:foetal calf serum

MTT:3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide

CC50:50 % cytotoxic concentration

HSV-1MC:Herpes simplex virus 1 McIntyre strain

TCID50:50 % tissue culture infective dose

CPE:cytopathic effect

IC50:50 % inhibitory concentration

PBMCs:peripheral blood mononuclear cells

PHA:phytohemagglutinin

References

Dr. Ewa Krawczyk

Department of Medical Microbiology

Medical University of Warsaw

5 Chalubinski St.

02-004 Warsaw

Poland

Phone: +48-22-622-0028

Fax: +48-22-628-2739

Email: ewakrawczyk@yahoo.com