Influence of Xanthohumol on the Binding Behavior of GABA_A Receptors and their Lateral Mobility at Hippocampal Neurons

Abstract

The influence of the xanthohumol from Humulus lupulus L. on the binding of muscimol-Alexa Fluor 532 (Mu-Alexa), a fluorescently labeled GABA_A receptor agonist, was studied by fluorescence correlation spectroscopy. An incubation of hippocampal neurons with 75 nM of xanthohumol increased the specific Mu-Alexa binding by approximately 17 %, which was selectively found in GABA_A receptor Mu-Alexa complexes with hindered lateral mobility [D_bound2 = (0.11 ± 0.03) μm²/s] as described with midazolam.

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