Planta Med 2006; 72(7): 656-658
DOI: 10.1055/s-2006-931609
Letter
© Georg Thieme Verlag KG Stuttgart · New York

Influence of Xanthohumol on the Binding Behavior of GABAA Receptors and their Lateral Mobility at Hippocampal Neurons

Oliver Meissner1 , Hanns Häberlein2
  • 1Department of Pharmaceutical Biology, University of Marburg, Marburg, Germany
  • 2Institute of Physiological Chemistry, University of Bonn, Bonn, Germany
Weitere Informationen

Publikationsverlauf

Received: April 26, 2005

Accepted: January 16, 2006

Publikationsdatum:
24. April 2006 (online)

Abstract

The influence of the xanthohumol from Humulus lupulus L. on the binding of muscimol-Alexa Fluor 532 (Mu-Alexa), a fluorescently labeled GABAA receptor agonist, was studied by fluorescence correlation spectroscopy. An incubation of hippocampal neurons with 75 nM of xanthohumol increased the specific Mu-Alexa binding by approximately 17 %, which was selectively found in GABAA receptor Mu-Alexa complexes with hindered lateral mobility [Dbound2 = (0.11 ± 0.03) μm2/s] as described with midazolam.

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Prof. Dr. Hanns Häberlein

Institute of Physiological Chemistry

University of Bonn

Nussallee 11

53115 Bonn

Germany

Fax: +49-228-732-416

eMail: haeberlein@institut.physiochem.uni-bonn.de