Planta Med 2006; 72(9): 771-785
DOI: 10.1055/s-2006-946682
Review
© Georg Thieme Verlag KG Stuttgart · New York

The Assessment of Plant Metabolite Profiles by NMR-Based Methodologies

Elaine Holmes1 , Huiru Tang2 , Yulan Wang1 , Christoph Seger3
  • 1Biological Chemistry, Biomedical Sciences Division, Faculty of Natural Science, Imperial College, London, U.K.
  • 2State Key Laboratory of Magnetic Resonance and Molecular and Atomic Physics, Wuhan Magnetic Resonance Centre, Wuhan Institute of Physics and Mathematics, The Chinese Academy of Sciences, Wuhan, P.R. China
  • 3Institute of Pharmacy/Pharmacognosy, Centre of Molecular Biosciences, University of Innsbruck, Innsbruck, Austria
Weitere Informationen

Publikationsverlauf

Received: April 13, 2006

Accepted: May 16, 2006

Publikationsdatum:
17. Juli 2006 (online)

Abstract

NMR-based metabolic profiling techniques can simultaneously track changes in many plant metabolites and have found a number of applications in both systems biology and biosafety. Together with multivariate statistical analyses, NMR spectroscopy has been successfully applied to the characterisation of various herbs and plant products for quality control, authentication, determining geographical origin and for detecting adulteration of products. Additionally, the metabolic consequences of plant extracts have been demonstrated in experimental animals and in man using NMR-based metabonomics approaches to characterise the response. Here the application of NMR spectroscopy and chemometric tools for analysing plant-based products and their metabolic consequences are considered with particular emphasis on deconvolving biological complexity and minimising confounding biological variability with analytical ‘noise’.

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  • 123 Griffin J L, Williams H J, Sang E, Clarke K, Rae C, Nicholson J K. Metabolic profiling of genetic disorders: A multi tissue 1H nuclear magnetic resonance spectroscopic and pattern recognition study into dystrophic tissue.  Anal Biochem. 2001;  293 16-21
  • 124 Ebbels T MD, Holmes E, Lindon J C, Nicholson J K. Evaluation of metabolic variation in normal rat strains from a statistical analysis of 1H NMR spectra of urine.  J Pharm Biomed Anal. 2004;  36 823-33
  • 125 Gavaghan McKee C L, Wilson I D, Nicholson J K. Metabolic phenotyping of nude and normal (Alpk:ApfCD, C57BL10J) mice.  J Proteome Res. 2006;  5 378-84
  • 126 Raamsdonk L M, Teusink B, Broadhurst D, Zhang N, Hayes A, Walsh M C. et al . A functional genomics strategy that uses metabolome data to reveal the phenotype of silent mutations.  Nat Biotechnol. 2001;  19 45-50
  • 127 Charlton A, Allnutt T, Holmes S, Chisholm J, Bean S, Ellis N. et al . NMR profiling of transgenic peas.  Plant Biotech J. 2004;  2 27-35
  • 128 Manetti C, Bianchetti C, Bizzarri M, Casciani L, Castro C, D’Ascenzo G. et al . NMR-based metabonomic study of transgenic maize.  Phytochemistry. 2004;  65 3187-98
  • 129 Wiklund S, Karlsson M, Antti H, Johnels D, Sjoestroem M, Wingsle G. et al . A new metabonomic strategy for analyzing the growth process of the poplar tree.  Plant Biotechnol J. 2005;  3 353-62
  • 130 Bailey N JC, Oven M, Holmes E, Nicholson J K, Zenk M H. Metabolomic analysis of the consequences of cadmium exposure in Silene cucubalus cell cultures via 1H NMR spectroscopy and chemometrics.  Phytochemistry. 2003;  62 851-8
  • 131 Ott K H, Aranibar N, Singh B, Stockton G W. Metabonomics classifies pathways affected by bioactive compounds. Artificial neural network classification of NMR spectra of plant extracts.  Phytochemistry. 2003;  62 971-85
  • 132 Solanky K S, Bailey N JC, Holmes E, Lindon J C, Davis A L, Mulder T PJ. et al . NMR-based metabonomic studies on the biochemical effects of epicatechin in the rat.  J Agric Food Chem. 2003;  51 4139-45
  • 133 Solanky K S, Bailey N J, Beckwith-Hall B M, Bingham S, Davis A, Holmes E. et al . Biofluid 1H NMR-based metabonomic techniques in nutrition research - metabolic effects of dietary isoflavones in humans.  J Nutr Biochem. 2005;  16 236-44
  • 134 Solanky K S, Bailey N JC, Beckwith-Hall B M, Davis A, Bingham S, Holmes E. et al . Application of biofluid 1H nuclear magnetic resonance-based metabonomic techniques for the analysis of the biochemical effects of dietary isoflavones on human plasma profile.  Anal Biochem. 2003;  323 197-204
  • 135 Daykin C A, Van Duynhoven J PM, Groenewegen A, Dachtler M, Van Amelsvoort J MM, Mulder T PJ. Nuclear magnetic resonance spectroscopic based studies of the metabolism of black tea polyphenols in humans.  J Agric Food Chem. 2005;  53 1428-34
  • 136 Wang Y, Tang H, Nicholson J K, Hylands P J, Sampson J, Holmes E. A metabonomic strategy for the detection of the metabolic effects of chamomile (Matricaria recutita L.) ingestion.  J Agric Food Chem. 2005;  53 191-196
  • 137 Fukusaki E, Kobayashi A. Plant metabolomics: potential for practical operation.  J Biosci Bioeng. 2005;  100 347-54
  • 138 Weckwerth W. Metabolomics in systems biology.  Ann Rev Plant Biol. 2003;  54 669-89
  • 139 Verpoorte R, Choi Y H, Kim H K. Ethnopharmacology and systems biology: a perfect holistic match.  J Ethnopharmacol. 2005;  100 53-6
  • 140 Bino R J, Hall R D, Fiehn O, Kopka J, Saito K, Draper J. et al . Potential of metabolomics as a functional genomics tool.  Trends Plant Sci. 2004;  9 418-25
  • 141 Jenkins H, Hardy N, Beckmann M, Draper J, Smith A R, Taylor J. et al . A proposed framework for the description of plant metabolomics experiments and their results.  Nat Biotechnol. 2004;  22 1601-6
  • 142 Kell D B. Metabolomics and system biology: making sense of the soup.  Curr Opin Microbiol. 2004;  7 296-307
  • 143 Jenkins H, Johnson H, Kular B, Wang T, Hardy N. Toward supportive data collection tools for plant metabolomics.  Plant Physiol. 2005;  138 67-77
  • 144 Lindon J C, Nicholson J K, Holmes E, Keun H C, Craig A, Pearce J T. et al . Summary recommendations for standardization and reporting of metabolic analyses.  Nat Biotechnol. 2005;  23 833-8

Dr. Christoph Seger

Institute of Pharmacy/Pharmacognosy

Centre of Molecular Biosciences

University of Innsbruck

Innrain 52

6020 Innsbruck

Austria

Telefon: +43-512-507-5344

Fax: +43-512-507-2939

eMail: christoph.seger@uibk.ac.at