Planta Med 2006; 72 - P_159
DOI: 10.1055/s-2006-949959

Study of metabolites in benzothiadiazole treated Arabidopsis using nuclear magnetic resonance spectroscopy and principal component analysis

HDT Thanh 1, 2, RC Puig 1, HK Kim 1, YH Choi 1, R Verpoorte 1
  • 1Pharmacognosy Department, Metabolomic Section, IBL, Leiden University, PO Box 9502, 2300RA Leiden, The Netherlands
  • 2Traditional Pharmacy Department, Hanoi Pharmacy University, 23 Le Thanh Tong, Hanoi, Vietnam
  • *Email address: d.hien@chem.leidenuniv.nl

The resistance against pathogen infection in Arabidopsis is associated with systemic acquired resistance (SAR) gene induction. After infection, mRNAs of PR1, PR2 and PR5 genes accumulate in a coordinate manner in tissues that become resistant to subsequent pathogen infection [1]. The SAR pathway also can be activated by benzothiadiazole (BTH) [2, 3]. BTH has been shown to activate SAR in tobacco [4], wheat and Arabidopsis [5]. A powerful tool for plant metabolite analysis is high-resolution nuclear magnetic resonance spectroscopy (NMR) combined with principal component analysis (PCA). We used NMR and PCA to study the changes in metabolite profile of Arabidopsis after treatment with BTH. The results shown clear changes in the metabolite profile in the polar fraction which contain compounds such as flavonoids and phenylpropanoids... but the metabolite profile in non-polar fraction did not show any difference between BTH treated and non-treated Arabidopsis.

Acknowledgements: Vietnamese overseas scholarship fund.

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