Planta Med 2006; 72 - P_185
DOI: 10.1055/s-2006-949985

Antioxidant activity of an aqueous fraction obtained from Indigofera truxillensis against ischemia-reperfusion-induced gastric lesions

V Barbastefano 1, E Farias-Silva 1, M Cola-Miranda 1, T Calvo 2, A Luiz-Ferreira 1, FO Pimentel 1, D de Paula Michelatto 1, CA Hiruma-Lima 3, W Vilegas 2, ARM Souza Brito 1
  • 1Departamento de Fisiologia e Biofísica, IB, UNICAMP, Campinas, SP, Brazil
  • 2Departamento de Química Orgânica, IQ, UNESP, Araraquara, SP, Brazil
  • 3Departamento de Fisiologia, IB, UNESP, Botucatu, SP, Brazil

The aqueous fraction (FAq) from the methanolic crude extract obtained from aerial parts of Indigofera truxillensis Kunth. (Fabaceae), a Brazilian savannah plant popularly known as “Indigo“, was investigated for its antioxidative proprieties in gastric lesions induced by ischemia-reperfusion (IR) in rats. Preliminar phytochemical screening showed that flavonoid glycosides are the major compounds present in this fraction. male Wistar rats (180–220g, n >4) were fasted during 24h, orally treated with FAq (100mg/kg), and submitted to gastric lesions by IR of the celiac artery. After, the stomach was removed, lesion areas were counted, gastric mucosa was scraped, homogenized, and frozen to posterior analysis of: a) DNA fragmentation, b) LPO and c) GSH content. FAq protected the gastric mucosa in 91% in IR model, but presented no significant changes over LPO and GSH content. Indeed, one of the antioxidative mechanisms observed in FAq was its capacity to protect cells from IR induced DNA fragmentation. Flavonoids should be responsible for this antiulcerogenic activity of FAq.

Acknowledgements: FAPESP, CNPq and CAPES.