Planta Med 2006; 72 - P_256
DOI: 10.1055/s-2006-950056

Effects of aqueous garlic extract on oxidant/antioxidant status in 32 D and 32 Dp cell lines

I Durak 1, A Sunguroglu 2, A Avci 1, E Devrim 1, IB Ergüder 1, GG Akay 2, P Ozkal 2, N Varol 2, D Akcora 2, B Altinok 3, D Gokmen 4
  • 1Ankara University School of Medicine, Department of Biochemistry, Sihhiye 06100 Ankara – Turkey
  • 2Ankara University School of Medicine, Department of Medical Biology, Sihhiye 06100 Ankara – Turkey
  • 3Ankara University Institute of Biotechnology, Ankara – Turkey
  • 4Ankara University School of Medicine, Department of Medical Statistics, Sihhiye 06100 Ankara – Turkey

It was aimed to investigate possible effects of aqueous garlic extract on oxidant/antioxidant status in 32 D (wild type mouse myeloid cell) and 32 Dp210 (BCR-ABL fusion gene (+) mouse myeloid cell) cell lines. Chronic myeloid leukemia (CML) is a myeloproliferative disorder that is characterized by Philedelphia (Ph) chromosome. This chromosome is caused by resiprocal translocation t(9;22)(q34;q11.2) which results in BCR-ABL fusion gene. We hypothesized that garlic could cause apoptosis in CML cells. Therefore, in this study, it is aimed to investigate possible antiproliferative and apoptotic effects of garlic on 32Dp210 (BCR-ABL fusion gene (+) mouse CML cell line) and 32D (wild type mouse myeloid cell line) cell lines.

For this aim, aqueous garlic extract (10% w/v) was added into the cell line media at 2 different final concentrations (0.4 and 1%). At the 0 time and 24, 48 and 72 hours later, oxidant (malondialdehyde-MDA level and xanthine oxidase-XO activity) and antioxidant (superoxide dismutase-SOD, glutathione peroxidase-GSH-Px and catalase-CAT activities) parameters were measured in the cell lines. It was observed that the garlic extract caused no change in XO and antioxidant enzyme activities but increased MDA level in the 32 D cell line. However, in the 32 Dp210 cell line treated by the garlic extract, significant increases in MDA level (1.63 nmol/million cells at 0 time vs. 4.05 nmol/million cells at the 72nd hour), XO and antioxidant enzyme activities were found. In conclusion, it has been suggested that garlic directly causes oxidant stress in 32 D cell line owing to its own oxidant ingredients and, that the oxidant stress created in 32 Dp210 cell line owing to garlic treatment might occur through increased XO activity and/or its own oxidant ingredients. Although antioxidant enzyme activities were found to increase in the 32 Dp210 cell line, it seemed that this compensatory change could not prevent the oxidant stress created. The oxidant potential of garlic extract might play part in the possible anticancer property of the garlic which was supposed by several investigators.