Planta Med 2006; 72 - P_332
DOI: 10.1055/s-2006-950132

In vitro effect of BA and NAA on growth and development of Pueraria candollei Grah. ex. Benth. var. mirifica

A Chawapun 1, S Dheeranupattana 1, A Jatisatienr 1, C Jatisatienr 1
  • 1Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand

Shoot of Pueraria candollei were cultured on MS medium with 0, 0.25, 0.5, 1.0 and 2.0mg/L BA found that medium supplemented with 0.5 and 1.0mg/L BA induced the greatest number of multiple shoots formation at 5.3 and 5.2 shoots/explant respectively for 12 weeks.

Shoot of Pueraria candollei from natural were cultured on MS medium supplemented with 1 and 2mg/L BA plus 1mg/L NAA. It was found that MS medium with 2mg/L BA plus 1mg/L NAA induced the greatest diameter of callus (2.0cm) for 4 weeks and shoots formation were induced the greatest number of 4.4 shoots/explant and root formation were induced the highest at 60% on MS medium with 1mg/L BA plus 1mg/L NAA for 10 weeks.

Acknowledgements: Chiang Mai University, Plant Tissue Culture Unit

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