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Planta Med 2006; 72(14): 1305-1310
DOI: 10.1055/s-2006-951692
Original Paper
Pharmacology
© Georg Thieme Verlag KG Stuttgart · New York

Wogonin Inhibits IL-6-Induced Angiogenesis via Down-Regulation of VEGF and VEGFR-1, not VEGFR-2

Chiu-Mei Lin1 , 2 , Hang Chang3 , 4 , Yeh-Hsu Chen6 , I-Hsing Wu2 , Jen-Hwey Chiu1 , 5 , 7
  • 1Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan, R.O.C.
  • 2Department of Emergency Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan, R.O.C.
  • 3Taipei City Hospital, Taipei, Taiwan, R.O.C.
  • 4College of Medicine, Taipei Medical University, Taipei, Taiwan, R.O.C.
  • 5Division of General Surgery, Department of Surgery, Veterans General Hospital, Taipei, Taiwan, R.O.C.
  • 6Division of Infectious diseases, Department of Internal Medicine, Chung-Ho Memorial Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan, R.O.C.
  • 7Research Center for Traditional Chinese Medicine. Veterans General Hospital, Taipei, Taiwan, R.O.C.
Further Information

Publication History

Received: May 3, 2006

Accepted: August 21, 2006

Publication Date:
06 October 2006 (online)

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Abstract

So far, no antiangiogenic activity of wogonin, a flavonoid, on human umbilical vein endothelial cells (HUVECs) has been demonstrated. The aim of this study was to investigate the effects of wogonin on IL-6-induced angiogenesis in HUVEC cultures and chorioallantoic membrane (CAM) neovascularization. The in vivo CAM model was applied to evaluate the percentage of new vessel formations, followed by measurement of endothelial migration and tube formation in HUVEC cultures. The results revealed that wogonin (10-8∼10-5M) concentration-dependently inhibited IL-6-induced angiogenesis. The signaling pathway was through down-regulation of the autocrine loop of VEGF and VEGFR-1, but not of VEGFR-2. Furthermore, the regulating loop of the IL-6 receptor complex was also attenuated via expression of sIL-6Rα and gp130, but not of IL-6/IL-6R binding density. We conclude that wogonin is a suppressive agent of the autoregulated loop of VEGF, VEGFR-1 and the IL-6 receptor complex.

Key words

Wogonin - angiogenesis - IL-6 - IL-6R - VEGF - VEGFR-1

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Jen-Hwey Chiu

Institute of Traditional Medicine

School of Medicine

National Yang-Ming University

155, Sec. 2, Li-Nong St.

Peitou

Taipei 112

Taiwan

Republic of China

Phone: +886-2-2826-7178

Fax: +886-2-2822-5044

Email: chiujh@mailsrv.ym.edu.tw