Action of (2→1)Fructo-oligopolysaccharide Fraction of Chlorophytum borivilianum against Streptozotocin-Induced Oxidative Stress

Abstract

A fructo-oligosaccharide was isolated from Chlorophytum borivilianum and identified as O-β-D-fructofuranosyl-(2→1)-(β-D-fructofuranosyl)_n-(2→1)-α-D-glucopyranoside (n = 5 - 30) using high-pressure anion exchange chromatography, MALDI-MS, NMR, GC, HPTLC and chemical analysis. The extract and the fructo-oligosaccharide were found to have significant antidiabetic activity with the blood sugar levels being 118.32 ± 3.56 and 110.21 ± 4.22, respectively, as compared to the control value of 231.25 ± 3.03 along with moderate antioxidant activity in streptozotocin-induced diabetic animals.

References

• 1 Sreevidya N, Kumar V, Kumar S, Sikarwar R LS. Utilization, depletion and conservation of Safed musli (Chlorophytum spp).  J Non-Timber Forest Prod. 2003;  10 155-7
• 2 Govindarajan R, Sreevidya N, Vijayakumar M, Thakur M, Dixit V K, Mehrotra S. et al . In vitro antioxidant activity of ethanolic extract of Chlorophytum borivilianum .  Nat Prod Sci. 2005;  11 165-9
• 3 Stahl E. Thin layer chromatography. 2nd edn London; George Allen & Unwin Ltd 1969: p 265
• 4 Archbold H K. Fructosans in the monocotyledons. A review.  New Phytol. 1940;  39 185-219
• 5 Barker S A, Bourne E J, Whiffen D H. Methods of Biochemical Analysis. Vol. 3 New York; Interscience 1956: p 212-3
• 6 Shiomi N. Structure of fructopolysaccharide (asparagosin) from the roots of asparagus (Asparagus officinalis L.)  New Phytol. 1993;  123 263-70
• 7 Rumessen J J, Bode S, Hamberg O, Gudmand-Hoyer E. Fructans of Jerusalem artichokes: intestinal transport, absorption, fermentation, and influence on blood glucose, insulin, and C-peptide responses in healthy subjects.  J Nutr. 1999;  129 402S-6S
• 8 Ashwell G. Colorimetric analysis of sugars. In: Ashwell G, editor Methods in enzymology. Vol. 3 New York; Academic Press 1957: p 75
• 9 Chatterton N J, Harrison P A, Thornley W R, Bennett J H. Purification and quantification of kestose (fructosylsucroses) by gel permeation and anion exchange chromatography.  Plant Physiol Biochem. 1989;  27 289-95
• 10 Stahl B, Linos A, Karas M, Hillenkamp F, Steup M. Analysis of fructooligosaccharides from higher plants by matrix assisted laser desorption/ionisation mass spectrometry.  Anal Biochem. 1997;  246 195-204
• 11 Ciucanu I, Costello C E. Elimination of oxidative degradation during the per-O-methylation of carbohydrates.  J Am Chem Soc. 2003;  125 16 213-9
• 12 Sasaki T, Matzy S, Sonal A. Effect of acetic acid concentration on the colour reaction in the o-toluidine boric acid method for blood glucose estimation.  Rinsho Kagaku. 1972;  1 346-53
• 13 Okhawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction.  Anal Biochem. 1979;  95 351-5
• 14 Marklund S, Marklund G. Involvement of superoxide anion radical and a convenient assay of superoxide dismutase.  Eur J Biochem. 1974;  47 469-74
• 15 Aebi H. Catalase. In: Bergmyer HU, editor Methods in enzymatic analysis. New York; Academic Press 1983: p 276-86
• 16 Lowry O H, Rosebrough N J, Farr A L, Randall R I. Protein determination using Folin ciocalteau reagent.  J Biol Chem. 1951;  193 438-48