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DOI: 10.1055/s-2006-951724
© Georg Thieme Verlag KG Stuttgart · New York
Coumarins from Cnidium monnieri and their Antiosteoporotic Activity
Publikationsverlauf
Received: August 25, 2005
Accepted: September 12, 2006
Publikationsdatum:
02. Januar 2007 (online)
![](https://www.thieme-connect.de/media/plantamedica/200701/lookinside/thumbnails/10.1055-s-2006-951724-1.jpg)
Abstract
Bioactivity-guided fractionation has led to the successful isolation of antiosteoporotic components, i. e., osthole, imperatorin and bergapten from an ethanolic extract of the fruits of Cnidium monnieri (L.) Cusson. Among them, osthole was determined as the major compound possessing antiosteoporotic activity. Further study showed that osthole not only promoted the proliferation and activity of alkaline phosphatase of osteoblasts in neonatal calvaria cultures, but also inhibited the bone resorption by decreasing the formation, differentiation and TRAP activity of osteoclasts derived from rat marrow cells.
Key words
Cnidium monnieri (L.) Cusson - Apiaceae - antiosteoporotic activity - osthole - imperatorin - bergapten - osteoblast - osteoclast
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References
- 1 Turner R T, Riggs B L, Spelsberg T C. Skeletal effects of estrogen. Endocr Rev. 1994; 15 275-300
- 2 Davison S, Davis S R. Hormone replacement therapy: current controversies. Clin Endocrinol. 2003; 58 249-61
- 3 Cassidy A. Dietary phytoestrogen and bone health. J Br Menopause Soc. 2003; 9 7-21
- 4 Li Q N, Liang N C, Wu T, WU Y, Xie H, Huang G D. et al . Effects of total coumarins of Fructus Cnidii on skeleton of ovariectomized rats. Acta Pharmacol Sin. 1994; 15 528-32
- 5 Guh J H, Yu S M, Ko F N, Wu T S, Teng C M. Antiproliferative effect in rat vascular smooth muscle cells by osthole, isolated from Angelica pubescens . Eur J Pharmacol. 1996; 298 191-7
- 6 Matsuda H, Tomohiro N, Ido Y, Kubo M. Anti-allergic effects of Cnidii monnieri fructus (dried fruits of Cnidium monnieri) and its major component, osthole. Biol Pharm Bull. 2002; 25 809-12
- 7 Chiou W F, Huang Y L, Chen C F, Chen C C. Vasorelaxing effect of coumarins from Cnidium monnieri on rabbit corpus cavernosum. Planta Med. 2001; 67 282-4
- 8 Xiang R D, Fu X H. Studies on the chemical constituens of Cnidium monnieri fruits. Chin Trad Herb Drugs. 1984; 15 14
- 9 Oh H, Kim J S, Song E K, Cho H, Kim D H, Park S E. et al . Sesquiterpenes with hepatoprotective activity from Cnidium monnieri on tacrine-induced cytotoxicity in Hep G2 cells. Planta Med. 2002; 68 748-9
- 10 Qin L P, Zhang Q Y, Tian Y P, Zheng H C, Huang M, Huang B K. Total coumarins from fruits of Cnidium monnieri (TCFC) inhibit the formation and differentiation of multinucleated osteoclast derived from rat marrow cells. Acta Pharmacol Sin. 2003; 24 181-6
- 11 Liu Z D, Zang H S, Ouyang Y P. Biological study of growth pattern of newborn rat calvaria osteoblastic cell in vitro . Acta Anatom Sin. 1995; 26 157-9
- 12 Zambonin Z A, Teti A, Primavera M V. Isolated osteoclasts in primary culture: first observation on structure and survival in culture media. Anat Embryol. 1982; 165 405-13
- 13 Owen T A, Aronow M, Shalhoub V, Barone L M, Wilming L, Tassinari M S. et al . Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationship in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracelluar matrix. J Cell Physiol. 1990; 143 420-30
- 14 Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72 248-54
- 15 Sugawara K, Hamada M, Hosoi S, Tamaoki T. A useful method to evaluate bone resorption inhibitors, using osteoclast-like multinucleated cells. Anal Biochem. 1998; 255 204-10
- 16 Harkar S, Razdan T K, Waight E S. Steroids, chromone and coumarins from Angelica officinalis . Phytochemistry. 1984; 23 419-26
- 17 Masuda T, Takasugi M, Anetai M. Psoralen and other linear furanocoumarins as phytoalexins in Glehnia littoralis . Phytochemistry. 1998; 47 13-6
- 18 Takahashi N, Yamana H, Yoshiki H, Roodman G D, Mundy G R, Jones S J. et al . Osteoclast-like cell formation and its regulation by osteotropic hormones in mouse bone marrow cultures. Endocrinology. 1988; 122 1373-82
- 19 Akatsu T, Takahashi N, Debari K, Morita I, Murota S, Nagata N. et al . Prostaglandins promote osteoclast-like cell formation by a mechanism involving cyclic adenosine 3′,5′-monophosphate in mouse bone marrow cell cultures. J Bone Miner Res. 1989; 4 29-35
- 20 Rodan G A, Martin T J. Role of osteoblasts in hormonal control of bone resorption - a hypothesis. Calcif Tissue Int. 1981; 33 349-51
- 21 Zhang Q Y, Qin L P, Tian Y P, Zheng H C, Huang B K, Liu Z D. et al . Effects of total coumarins from the fruits of Cnidium monnieri on the production of NO, IL-1 and IL-6 from osteoblast in neonatal rat calvaria cultures. Chin Pharm J. 2003; 38 345-8
- 22 Chian T. The experimental studies of extracts of Fructus Cnidii and Herb Epimedii on sex period of ovariectomized rat and mice. Folia Pharmacol Jpn. 1959; 55 153
- 23 Michigami T, Ihara-Watanabe M, Yamazaki M. Receptor activator of nuclear factor kappa B ligand (RANKL) is a key molecule of osteoclast formation for bone metastasis in a newly developed model of human neuroblastoma. Cancer Res. 2001; 61 1637-44
- 24 Ojala T, Vuorela P, Kiviranta J, Vuorela H, Hiltunen R. A bioassay using Artemia salina for detecting phototoxicity of plant coumarins. Planta Med. 1999; 65 715-8
Prof. Dr. Luping Qin
Department of Pharmacognosy
School of Pharmacy
Second Military Medical University
200433 Shanghai
People’s Republic of China
Telefon: +86-21-2507-0394
Fax: +86-21-2507-0394
eMail: lpqin@smmu.edu.cn
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