Planta Med 2007; 73(1): 67-70
DOI: 10.1055/s-2006-951746
Original Paper
Biochemistry and Molecular Biology
© Georg Thieme Verlag KG Stuttgart · New York

Molecular Authentication of Alisma orientale by PCR-RFLP and ARMS

Xuexia Li1 , Xiaoyu Ding1 , Bihai Chu1 , Ge Ding1 , Sun Gu1 , Liang Qian1 , Ying Wang1 , Qi Zhou1
  • 1Jiangsu Key Laboratory for Biodiversity & Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, P. R. China
Further Information

Publication History

Received: May 31, 2006

Accepted: October 13, 2006

Publication Date:
15 November 2006 (online)

Abstract

As a widely used medicinal plant, Alisma orientale is always a possible target for fraudulent labeling. The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (nrDNA) of the six species of genus Alisma were sequenced, and two variant sites were found to be specific for A. orientale. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis and amplification refractory mutation system (ARMS) analysis were applied to the ITS region for the identification of A. orientale. A restriction site for PstI useful for PCR-RFLP analysis was detected and a pair of diagnostic primers DFZX-JB02S and DFZX-JB02X were designed for ARMS.

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Professor Xiaoyu Ding

Jiangsu Key Laboratory for Biodiversity & Biotechnology

College of Life Sciences

Nanjing Normal University

Nanjing

People’s Republic of China

Email: dingxynj@263.net