Abstract
Chromatographic separation of a methanolic extract from Iris pseudopumila rhizomes led to the isolation of two new natural compounds, irilone 4′-O-[β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside] and isoscutellarein 6-C-β-D-glucopyranoside, along with 9 isoflavonoids and 4 flavonoids. Their structures were determined on the basis of spectroscopic evidence. Isolated compounds were evaluated for their antioxidant activity using the luminol-dependent chemiluminescence assay, with quercetin as a positive control. Irilone 4′-O-β-D-glucopyranoside and kaempferol 3-O-[α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside] showed significant antioxidant activity.
References
-
1 Baroni E. Guida botanica d′Italia. Bologna; Cappelli 1977: 412-3
-
2
Orhan I, Nasim S, Sener B, Ayanoglu F, Ozguven M, Choudhary M I. et al .
Two isoflavones and bioactivity spectrum of the crude extracts of Iris germanica rhizomes.
Phytother Res.
2003;
17
575-7
-
3
Bonfils J P, Pinguet F, Culine S, Sauvaire Y.
Cytotoxicity of iridals, triterpenoids from Iris, on human tumor cell lines A2780 and K562.
Planta Med.
2001;
67
79-81
-
4
Williams C, Harborne J, Colasante M.
Flavonoid and xanthone patterns in bearded Iris species and the pathway of chemical evolution in the genus.
Biochem Syst Ecol.
1997;
25
309-25
-
5
Demmig-Adams B, Adams WW 3 rd.
Antioxidants in photosynthesis and human nutrition.
Science.
2002;
298
2149-53
-
6
Finkel T, Holbrook N J.
Oxidants, oxidative stress and the biology of ageing.
Nature.
2000;
408
239-47.
-
7
Gobbo-Neto L, Santos M D, Kanashiro A, Almeida M C, Lucisano-Valim Y M, Lopes J L. et al .
Evaluation of the anti-inflammatory and antioxidant activities of di-C-glucoflavones from Lychnophora ericoides (Asteraceae).
Planta Med.
2005;
71
3-6
-
8
DeChatelet L R, Long G D, Shirley P S, Bass D A, Thomas M J, Henderson F W. et al .
Mechanism of the luminol-dependent chemiluminescence of human neutrophils.
J Immunol.
1982;
129
1589-93
-
9
Vuotto M L, Miranda R, Ritieni A, Basile A, Ricciardi L, Di Prisco R. et al .
Improvement of (+)-catechin inhibitory activity on human PMN respiratory burst by (+)-3-O-propionyl and (-)-3-O-valeryl substitution.
J Pharm Pharmacol.
2003;
55
399-405
-
10
Williamson G, Manach C.
Bioavailability and bioefficacy of polyphenols in humans. II. Review of 93 intervention studies.
Am J Clin Nutr.
2005;
81
S243-55
-
11
Atta-Ur-Rahman , Nasim S, Baig I, Orhan I, Sener B, Ayanoglu F. et al .
Isoflavonoid glycosides from the rhizomes of Iris germanica
.
Helv Chim Acta.
2003;
86
3354-62
-
12 Agrawal P K. Carbon-13 NMR of flavonoids. London; Elsevier 1989
-
13
Ghanim A, Prakash L, Zaman A, Kidwai A R.
Isolation of iridin from Iris kamaonensis
.
Indian J Chem.
1963;
1
230-1
-
14
Prakash L, Zaman A, Kidway A R.
Chemical examination of Iris nepalensis. III. Isolation and structure of irisolidone.
J Org Chem.
1965;
30
3561-2
-
15
Tsukida K, Saiki K, Ito M.
New isoflavone glycosides from Iris florentina
.
Phytochemistry.
1973;
12
2318-9
Dr. Daniela Rigano
Department of Chemistry of Natural Products
University of Naples ”Federico II”
via D. Montesano 49
80131 Naples
Italy
Phone: +39-081-678-546
Fax: +39-081-678-552
Email: drigano@unina.it
