Inhibition of Trypanosoma cruzi Growth in vitro by Solanum Alkaloids: A Comparison with Ketoconazole

Abstract

The glycoalkaloids α-chaconine, α-solamargine, α-so-lanine, solasonine, sycophantine, and tomatine, as well as the aglycones demissidine, solanidine, solanocapsine, solasodine, tomatidine, and veratrine were tested as growth inhibitors of Trypanosoma cruzi, strain EP, in LIT medium. Their activity was compared with the antifungal ketoconazole. Glycoalkaloids containing α-chacotriose showed trypanolytic activity against the epimastigote form and trypanocidal activity against the bloodstream and metacyclic trypomastigote form of Trypanosoma cruzi in culture medium in micromolar concentrations. Ketoconazole showed a lower activity, at the same concentrations of α-chaconine and α-solamargine. The observations indicate that the initial target of the compound is at the membrane level with a concomitant change in the parasite morphology. Moreover, internal compartments of the parasites were observed to be affected by the drugs, revealing the dissolution of some organelles as mitocondrias and glycosomes.

Key words

Solanum alkaloids - steroidal glycoalkaloids - Trypanosoma cruzi - ketoconazole - trypanolytic effects - trypanocidal effects