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Planta Med 1998; 64(4): 387-388
DOI: 10.1055/s-2006-957461
Letters

© Georg Thieme Verlag Stuttgart · New York

Recombinant Expression of Alliin Lyase from Garlic (Allium sativum) in Bacteria and Yeasts

Robert Weik1 , Andrej Francky2 , Gerald Striedner1 , Peter Raspor2 , Karl Bayer1 , Diethard Mattanovich1
  • 1Institut für Angewandte Mikrobiologie, Universität für Bodenkultur Wien, Austria
  • 2Biotechnical Faculty, University of Ljubljana, Slovenia
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Publikationsverlauf

1997

1998

Publikationsdatum:
04. Januar 2007 (online)

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Abstract

Recombinant garlic alliin lyase was produced in Escherichia coli, Saccharomyces cerevisiae, and Pichia pastoris. A cDNA clone was obtained from garlic bulbs by PCR and introduced into suitable bacterial and yeast expression vectors. The recombinant alliin lyase forms inclusion bodies in all three host organisms, which are deposited in the cytoplasm. After cell lysis and harvesting by centrifugation, the inclusion bodies were solubilized in Zwittergent 3-14 solution and refolded by step-wise dilution. Specific alliin lyase activity could be recovered by this procedure.