Antioxidant and Lipophilic Constituents of Tinospora crispa

Abstract

TLC autographic assays revealed in the CH₂Cl₂ extract of Tinospora crispa Miers (Menispermaceae) the presence of three compounds exhibiting antioxidant and radical scavenging properties towards β-carotene and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. They were isolated and identified as N-cis-feruloyltyramine (4), (N-trans-feruloyltyramine (6) and secoisolariciresinol (5). When tested in dilution assays on the reduction of the DPPH radical, these 3 compounds proved to be more active than the synthetic antioxidant butylhydroxytoluene (BHT). Further investigation of the CH₂Cl₂ extract led to the isolation of vanillin, syringin, the alkaloid N-formylnornuciferin and the diterpene derivatives borapetosides B and C. In addition, a LC/UV/MS analysis enabled the on-line identification of borapetoside F and N-formylannonain.

Key words

Tinospora crispa - Menispermaceae - antioxidants - free radical scavengers - secoisolariciresinol - N-feruloyltyramine - LC/MS