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DOI: 10.1055/s-2006-957512
© Georg Thieme Verlag Stuttgart · New York
Conjugates of Tremella Polysaccharides with Microbeads and their TNF-Stimulating Activity
Publikationsverlauf
1997
1998
Publikationsdatum:
01. Februar 2007 (online)

Abstract
A mannan (A) and a heteroglycan (D) were prepared by partial acidic hydrolysis of T3, a major polysaccharide fraction of the fungus Tremella fuciformis Berk. Methylation analysis of A and D showed that they contained a 1→3 linked mannosyl main chain, to which were linked different side chains at positions 2, 4, or 6 of the mannosyl residues. A and D were conjugated to albumin microbeads (AM) by reductive amination. The conjugates showed significant cytokine-stimulating activity in vitro whereas unconjugated AM had no activity. Not conjugated A and D showed cytokine-stimulating activity only in about 100 times higher concentrations.
Key words
Tremella fuciformis - Tremellaceae - mannan heteroglycan - microbeads conjugate - cytokine-stimulating activity