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Planta Med 1998; 64(6): 551-554
DOI: 10.1055/s-2006-957512
Papers
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Conjugates of Tremella Polysaccharides with Microbeads and their TNF-Stimulating Activity

Qipin Gao1 , 2 , Gørin Berntzen1 , Ruizhi Jiang2 , Mette Kjær Killie1 , Rolf Seljelid1
  • 1Department of Experimental Pathology, IMB, University of Tromsø, Tromsø, Norway
  • 2Academy of Chinese Traditional Medicine and Material Medica of Jilin Province, Changchun, P. R. China
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Publication History

1997

1998

Publication Date:
01 February 2007 (online)

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Abstract

A mannan (A) and a heteroglycan (D) were prepared by partial acidic hydrolysis of T3, a major polysaccharide fraction of the fungus Tremella fuciformis Berk. Methylation analysis of A and D showed that they contained a 1→3 linked mannosyl main chain, to which were linked different side chains at positions 2, 4, or 6 of the mannosyl residues. A and D were conjugated to albumin microbeads (AM) by reductive amination. The conjugates showed significant cytokine-stimulating activity in vitro whereas unconjugated AM had no activity. Not conjugated A and D showed cytokine-stimulating activity only in about 100 times higher concentrations.

Key words

Tremella fuciformis - Tremellaceae - mannan heteroglycan - microbeads conjugate - cytokine-stimulating activity